Crystal structure of the human mitochondrial PRELID1K58V-TRIAP1 complex with PS

Structural determinants of lipid specificity within Ups/PRELI lipid transfer proteins.

PRELI domain-containing protein 1, mitochondrial

25 kDa protein of relevant evolutionary and lymphoid interest,Px19-like protein

Protein 15E1.1,WF-1,p53-inducible cell-survival factor,p53CSV

GSSHHHHHHSDQMVKYFLGQSVLRSSWDQVFAAFWQRYPNPYSKHVLTEDIVHREVTPDQKLLSRRLLTVTNRMPRWAER
LFPANVAHSVYVLEDSIVDPQNQTMTTFTWNINHARLMVVEERSVYSVNSDNSGWTEIRREAWVSSSLFGVSRAVQEFGL
ARFKSNVTKTMKGFEYILAKLQGEA

MAHHHHHHVDDDDKMNSVGEACTDMKREYDQCFNRWFAEKFLKGDSSGDPCTDLFKRYQQCVQKAIKEKEIPIEGLEFMG
HGKEKPENSS

100 mM sodium cacodylate pH 6.5
40% PEG 300

Medical Research Council (United Kingdom)

Lipidomics and native mass spectrometry used to confirm substrate binding. DOPS was exchanged to 25% in the PRELIK58V sample (see supplementary materials) upon incubation of 10X (6:3) DDM-DOPS.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Mitochondrial lipid transport, Complex, Phosphatidylserine bound, PA transport, Apoptosis, LIPID TRANSPORT

MVKYFLGQSVLRSSWDQVFAAFWQRYPNPYSKHVLTEDIVHREVTPDQKLLSRRLLTKTNRMPRWAERLFPANVAHSVYV
LEDSIVDPQNQTMTTFTWNINHARLMVVEERCVYCVNSDNSGWTEIRREAWVSSSLFGVSRAVQEFGLARFKSNVTKTMK
GFEYILAKLQGEA