Flavin Analogue Sheds Light on Light-Oxygen-Voltage Domain Mechanism

A Noncanonical Chromophore Reveals Structural Rearrangements of the Light-Oxygen-Voltage Domain upon Photoactivation.

GAMDYSLVKALQTAQQNFVISDPSIPDNPIVYASQGFLTLTGYALSEVLGRNCRFLQGPETDPKAVEKVRKGLERGEDTT
VVLLNYRKDGSTFWNQLFIAALRDGEGNVVNYLGVQCKVSEDYAKAFLKN

GAMDYSLVKALQTAQQNFVISDPSIPDNPIVYASQGFLTLTGYALSEVLGRNCRFLQGPETDPKAVEKVRKGLERGEDTT
VVLLNYRKDGSTFWNQLFIAALRDGEGNVVNYLGVQCKVSEDYAKAFLKNEEN

0.1 M Na citrate,
0.3 M NH4 chloride,
15-20% PEG 2000

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

LOV Domain, FMN, Dark grown, fluorescence, light sensing, transcription factor PAS domain, Ochronomas danica, transcription

DYSLVKALQTAQQNFVISDPSIPDNPIVYASQGFLTLTGYALSEVLGRNCRFLQGPETDPKAVEKVRKGLERGEDTTVVL
LNYRKDGSTFWNQLFIAALRDGEGNVVNYLGVQCKVSEDYAKAFLKN

DYSLVKALQTAQQNFVISDPSIPDNPIVYASQGFLTLTGYALSEVLGRNCRFLQGPETDPKAVEKVRKGLERGEDTTVVL
LNYRKDGSTFWNQLFIAALRDGEGNVVNYLGVQCKVSEDYAKAFLKNEEN