BP0997, GH138 enzyme targeting pectin rhamnogalacturonan II

alpha-D-galacturonic acid; D-galacturonic acid; galacturonic acid; ALPHA D-GALACTURONIC ACID

beta-D-galacturonic acid; D-galacturonic acid; galacturonic acid

Structural and functional analyses of glycoside hydrolase 138 enzymes targeting chain A galacturonic acid in the complex pectin rhamnogalacturonan II.

MRRYNTGLFLLSMVMLAGCGKPEVNVVMTGDMTTRLAFAGEQLKQALVEKGYEVNQTTDEKEIGGGKRSIYLNLLNDTTK
KNKERFDISTKGKNTYVTGYDGNGIIYGCRELIDQLDQSGTMDFKPVSDAPEMVLRGACIGLQKTTYLPGHAVYEYPYTP
ESFPWFYDKERWIKYLDMMVENRMNSLYLWNGHPFASLVKLKDYPFALEVDEETFKKNEEMFSFLTTEAEKRGIFVIQMF
YNIIVSKPFADHYGIKTQDRNRPITPLISDYTRKSVAAFIEKYPNVGLLVCLGEAIGTYEEDVEWFTKTIIPGIKDGLKV
LGRTDEPPVLVRAHDTDCKMVIDAALPLYKNLYTMHKYNGSSLTTYEPRGPWAKIHKDLSSLGSVHISNVHILANLEPWR
WSSPDFIQKSVKAMHSVHGANALHIYPQANYWDWPYTADKLANGEREEQVYRDWAWYKAWGRYAWKADRNRLEEIKYWDK
QFGDFYGIPAEMADNIRIAYEESGEIAPKLLRRFGITEGNRQTLLLGMFMSQFVNPYKYTIHYGFYESCGPGGEKLIEYV
EKEWKKQPHVGELPLDIINQVIEHGDKAVAAIDKVVSSAKKNSDELRRLQNDMHCYREYAYAFYYKVKAAQHVLNYHWGK
NMDELDKAVPLMEESLKHYTKLVDLTKDTYLFANSMQTAQRRIPIGGDDGNNKTWSEMLVHYKAELYNFKENIEMLKDKK
VRKCVEVTPLKEADVKILNNLTKVKIEKGAKIFSNIDGGIDAIAKEITGLTGFVFNGEKQRDDATTIEFECSSPVTMLVA
YFKDDHRKFAKAPRLESDASANDYGQAEPVLTNALHVKGVALADIYPYKFKAGRHTLILPKGYCGVLGFTEDKIKERDVA
LEGGADAPDWLFY

16 % PEG 8000, 100 mM Tris pH 8.4, 9% Butanol

HYDROGENS HAVE BEEN ADDED IN THE RIDING
POSITIONS

Rahmnogalacturonan II, pectin, glycoside hydrolase family 38, HYDROLASE