Solution NMR structure of the tetramethylrhodamine (TMR) aptamer 3 in complex with 5-TAMRA
Structure of an RNA aptamer in complex with the fluorophore tetramethylrhodamine.
NMR resonance assignments for the tetramethylrhodamine binding RNA aptamer 3 in complex with the ligand 5-carboxy-tetramethylrhodamine.
in vitro transcription vector pT7-TP(deltai)
structures with the least restraint violations
3D f1-13C,15N filtered 1H-13C NOESY aliphatic
3D f1-13C,15N filtered 1H-13C NOESY aromatic
1.2 mM unlabeled TMR3 (48-MER), 1.4 mM unlabeled 5-TAMRA, 100% D2O
1.2 mM [U-13C; U-15N] TMR-3_48nt, 1.4 mM unlabeled 5-TAMRA, 100% D2O
1.2 mM [U-15N] TMR3 (48-MER), 1.4 mM unlabeled 5-TAMRA, 95% H2O/5% D2O
1.2 mM [U-13C; U-15N]-Ade,Cyt TMR3 (48-MER), 1.4 mM unlabeled 5-TAMRA, 95% H2O/5% D2O
1.2 mM [U-13C; U-15N]-Gua,Ura TMR3 (48-MER), 1.4 mM unlabeled 5-TAMRA, 95% H2O/5% D2O
1.2 mM [U-13C; U-15N]-Ade,Cyt TMR3 (48-MER), 1.4 mM unlabeled 5-TAMRA, 100% D2O
1.2 mM [U-13C; U-15N]-Gua,Ura TMR3 (48-MER), 1.4 mM unlabeled 5-TAMRA, 100% D2O
1.2 mM [U-13C; U-15N]-Ade TMR3 (48-MER), 1.4 mM unlabeled 5-TAMRA, 95% H2O/5% D2O
Fluorescence spectroscopy NMR titrations
RNA Aptamer, RNA-Ligand complex, three-way junction, RNA