DNA-bound pRN1 helix bundle domain with ATP and magnesium in the interaction buffer
A Small Helical Bundle Prepares Primer Synthesis by Binding Two Nucleotides that Enhance Sequence-Specific Recognition of the DNA Template.
Sequence of the DNA template from the Sulfolobus islandicus pRN1 plasmid
DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3')
DNA-bound pRN1 helix bundle domain with ATP and magnesium in interaction buffer at pH 7.0
TVVEFEELRKELVKRDSGKPVEKIKEEICTKSPPKLIKEIICENKTYADVNIDRSRGDWHVILYLMKHGVTDPDKILELL PRDSKAKENEKWNTQKYFVITLSKAWSVVKKYLEA
DNA-bound pRN1 helix bundle domain with ATP
Experimental conditions for quaternary arrangement assignment and structure determination 25mM phosphate buffer; 50mM NaCl
Complementary experimental conditions for quaternary structure determination 25mM phosphate buffer; 50mM NaCl
Complementary experimental conditions for quaternary arrangement assignment 25mM acetate buffer; 100mM NaCl
1.1 mM [U-99% 13C; U-99% 15N] DNA-bound pRN1 helix bundle domain with ATP, 1.1 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O
13C-15N-labeled-hbd bound to DNA with 10mM ATP and magnesium in interaction buffer
0.9 mM [U-99% 13C; U-99% 15N] DNA-bound pRN1 helix bundle domain with ATP, 0.9 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 100% D2O
13C-15N-labeled-hbd bound to DNA with 10mM ATP and magnesium in interaction buffer (100% D2O)
0.7 mM [U-100% 13C] DNA-bound pRN1 helix bundle domain with ATP, 0.7 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 100% D2O
13C-labeled-hbd bound to DNA with 10mM ATP and magnesium in interaction buffer (100% D2O)
1.0 mM DNA-bound pRN1 helix bundle domain with ATP, 1.0 mM DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3'), 90% H2O/10% D2O
1H-hbd bound to DNA with 10mM ATP and magnesium in interaction buffer
Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman
pRN1 primase, dinucleotide formation, quaternary structure of the helix bundle domain, synergistic effect, DNA BINDING PROTEIN