Serum paraoxonase-1 by directed evolution with the L69G/H134R/F222S/T332S mutations

Enzyme Evolution: An Epistatic Ratchet versus a Smooth Reversible Transition.

1. There is no entry for this protein in the Uniprot, since the protein is a chimera (recombinant protein) of four genes (human, rabbit, mouse and rat).

2. The first 17 aa at the N-term is missing due to lack of electron density.

MAKLTALTLLGMGLALFDRQKSSFQTRFNVHREVTPVELPNCNLVKGIDNGSEDLEILPNGLAFISSGGKYPGIMSFDPD
KSGKILLMDLNEKEPAVSELEIIGNTLDISSFNPHGISTFIDDDNTVYLLVVNRPGSSSTVEVFKFQEEEKSLLHLKTIR
HKLLPSVNDIVAVGPEHFYATNDHYFIDPYLKSWEMHLGLAWSFVTYYSPNDVRVVAEGFDSANGINISPDGKYVYIAEL
LAHKIHVYEKHANWTLTPLRVLSFDTLVDNISVDPVTGDLWVGCHPNGMRIFFYDAENPPGSEVLRIQDILSEEPKVTVV
YAENGTVLQGSSVAAVYKGKLLIGTVFHKALYCDL

0.1M Bis-Tris Propane pH 6.5, 0.2M Sodium Bromide and 20% PEG 3350

beta-propeller, Lactonase, HDL, Organophosphate-hydrolase, HYDROLASE