PURPLE ACID PHYTASE FROM WHEAT ISOFORM B2 - COMPLEX WITH INOSITOL HEXASULPHATE

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

Structure of a cereal purple acid phytase provides new insights to phytate degradation in plants.

EPASTLEGPSRPVTVPLREDRGHAVDLPDTDPRVQRRVTGWAPEQIAVALSAAPTSAWVSWITGDFQMGGAVKPLDPGTV
GSVVRYGLAADSLVREATGDALVYSQLYPFEGLQNYTSGIIHHVRLQGLEPGTKYYYQCGDPSIPGAMSAVHAFRTMPAV
GPRSYPGRIAVVGDLGLTYNTTSTVEHMASNQPDLVLLLGDVSYANLYLTNGTGTDCYSCSFAKSTPIHETYQPRWDYWG
RYMEPVTSSTPMMVVEGNHEIEQQIGNKTFAAYSARFAFPSMESESFSPFYYSFDAGGIHFIMLAAYADYSKSGEQYRWL
EKDLAKVDRSVTPWLVAGWHAPWYSTYKAHYREAECMRVAMEELLYSYGLDIVFTGHVHAYERSNRVFNYTLDPCGAVHI
SVGDGGNREKMATTHADDPGRCPEPMSTPDAFMGGFCAFNFTSGPAAGSFCWDRQPDYSAYRESSFGHGILEVKNETHAL
WKWHRNQDLYQGAVGDEIYIVREPERCLLKHHHHHH

0.2M SODIUM THIOCYANATE; 20% (W/V) PEG 3350

Biotechnology and Biological Sciences Research Council

6GIT CONTAINS THE SAME PROTEIN IN COMPLEX WITH PHOSPHATE

EC 3.1.3.26, HYDROLASE, PURPLE ACID PHYTASE

EPASTLEGPSRPVTVPLREDRGHAVDLPDTDPRVQRRVTGWAPEQIAVALSAAPTSAWVSWITGDFQMGGAVKPLDPGTV
GSVVRYGLAADSLVREATGDALVYSQLYPFEGLQNYTSGIIHHVRLQGLEPGTKYYYQCGDPSIPGAMSAVHAFRTMPAV
GPRSYPGRIAVVGDLGLTYNTTSTVEHMASNQPDLVLLLGDVSYANLYLTNGTGTDCYSCSFAKSTPIHETYQPRWDYWG
RYMEPVTSSTPMMVVEGNHEIEQQIGNKTFAAYSARFAFPSMESESFSPFYYSFDAGGIHFIMLAAYADYSKSGEQYRWL
EKDLAKVDRSVTPWLVAGWHAPWYSTYKAHYREAECMRVAMEELLYSYGLDIVFTGHVHAYERSNRVFNYTLDPCGAVHI
SVGDGGNREKMATTHADDPGRCPEPMSTPDAFMGGFCAFNFTSGPAAGSFCWDRQPDYSAYRESSFGHGILEVKNETHAL
WKWHRNQDLYQGAVGDEIYIVREPERCLLK