Crystal structure of haspin in complex with 5-bromotubercidin

Halogen-Aromatic pi Interactions Modulate Inhibitor Residence Times.

Germ cell-specific gene 2 protein,H-haspin,Haploid germ cell-specific nuclear protein kinase

MHHHHHHSSGVDLGTENLYFQSMGECSQKGPVPFSHCLPTEKLQRCEKIGEGVFGEVFQTIADHTPVAIKIIAIEGPDLV
NGSHQKTFEEILPEIIISKELSLLSGEVCNRTEGFIGLNSVHCVQGSYPPLLLKAWDHYNSTKGSANDRPDFFKDDQLFI
VLEFEFGGIDLEQMRTKLSSLATAKSILHQLTASLAVAEASLRFEHRDLHWGNVLLKKTSLKKLHYTLNGKSSTIPSCGL
QVSIIDYTLSRLERDGIVVFCDVSMDEDLFTGDGDYQFDIYRLMKKENNNRWGEYHPYSNVLWLHYLTDKMLKQMTFKTK
CNTPAMKQIKRKIQEFHRTMLNFSSATDLLCQHSLFK

51-63% MPD and 0.1M SPG buffer, pH 6.0-6.5

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

kinase, inhibitors, slow off-rate, kinetics, halogen, Structural Genomics, Structural Genomics Consortium, SGC, TRANSFERASE

GECSQKGPVPFSHCLPTEKLQRCEKIGEGVFGEVFQTIADHTPVAIKIIAIEGPDLVNGSHQKTFEEILPEIIISKELSL
LSGEVCNRTEGFIGLNSVHCVQGSYPPLLLKAWDHYNSTKGSANDRPDFFKDDQLFIVLEFEFGGIDLEQMRTKLSSLAT
AKSILHQLTASLAVAEASLRFEHRDLHWGNVLLKKTSLKKLHYTLNGKSSTIPSCGLQVSIIDYTLSRLERDGIVVFCDV
SMDEDLFTGDGDYQFDIYRLMKKENNNRWGEYHPYSNVLWLHYLTDKMLKQMTFKTKCNTPAMKQIKRKIQEFHRTMLNF
SSATDLLCQHSLFK