Structure of A3_A3, an artificial bi-domain protein based on two identical alphaRep A3 domains

Ligand-induced conformational switch in an artificial bidomain protein scaffold.

Bi-domain artificial protein alphaRep display inducible cooperativity

MRGSHHHHHHTENLYFQGAADPEKVEMYIKNLQDDSYYVRRAAAYALGKIGDERAVEPLIKALKDEDAWVRRAAADALGQ
IGDERAVEPLIKALKDEDGWVRQSAAVALGQIGDERAVEPLIKALKDEDWFVRIAAAFALGEIGDERAVEPLIKALKDED
GWVRQSAADALGEIGGERVRAAMEKLAETGTGFARKVAVNYLETHKSLISGGGGSGGGGDPEKVEMYIKNLQDDSYYVRR
AAAYALGKIGDERAVEPLIKALKDEDAWVRRAAADALGQIGDERAVEPLIKALKDEDGWVRQSAAVALGQIGDERAVEPL
IKALKDEDWFVRIAAAFALGEIGDERAVEPLIKALKDEDGWVRQSAADALGEIGGERVRAAMEKLAETGTGFARKVAVNY
LETHKSLIS

Sodium-citrate (0.1M)
Ammonium Sulfate (2.4M)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

alphaRep, artificial protein, chimera, bidomain, BIOSYNTHETIC PROTEIN