Crystal structure of the BEC1054 RNase-like effector from the fungal pathogen Blumeria graminis
AAAYWDCDGTEIPERNVRAAVVLAFNYRKESFHGYPATFIIGSTFSGVGEVRQFPVEDSDANWQGGAVKYYILTNKRGSY LEVFSSVGSGNKCTFVEG
Purified CSEP0064/BEC1054 was dialysed into crystallisation buffer (10 mM Tris, 150 mM NaCl, pH 7.0) and concentrated to 10 mg/ml for crystallisation. Commercially available solution conditions for crystallisation (Hampton Research,CA, USA) were screened. The protein was combined with the mother liquor on a 1:1 ratio in 200 nl drops. Crystals obtained in 0.1 M sodium acetate buffer pH 5.0, supplemented with 30% PEG 4000, 0.4 M (NH4)2SO4 were cryoprotected with 30% glycerol and flash frozen for data collection.
HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Blumeria graminis; effector; CSEP0064/BEC1054; RIP; Bimolecular Fluorescence Complementation; split YFP; RALPH; RNA-binding protein; RNase structure; fungal pathogen, RNA BINDING PROTEIN