Crystal structure of R. ruber ADH-A, mutant F43H

Directed Evolution of Alcohol Dehydrogenase for Improved Stereoselective Redox Transformations of 1-Phenylethane-1,2-diol and Its Corresponding Acyloin.

MKAVQYTEIGSEPVVVDIPTPTPGPGEILLKVTAAGLCHSDIHVMDMPAAQYAYGLPLTLGHEGVGTVAELGEGVTGFGV
GDAVAVYGPWGCGACHACARGRENYCTRAADLGITPPGLGSPGSMAEYMIVDSARHLVPIGDLDPVAAAPLTDAGLTPYH
AISRVLPLLGPGSTAVVIGVGGLGHVGIQILRAVSAARVIAVDLDDDRLALAREVGADAAVKSGAGAADAIRELTGGQGA
TAVFDFVGAQSTIDTAQQVVAVDGHISVVGIHAGAHAKVGFFMIPFGASVVTPYWGTRSELMEVVALARAGRLDIHTETF
TLDEGPAAYRRLREGSIRGRGVVVPTSHHHHH

17% Polyacrylic acid 5100
0.1 M Tris pH 8
4 mM NAD+
20 mM MgCl2
100 mM phenylethandiol
7.5 mg/ml ADHA-mutant

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

alcohol dehydrogenase mutant variant, NADH-dependent, Zn2+-dependent, Rossmann fold, OXIDOREDUCTASE

MKAVQYTEIGSEPVVVDIPTPTPGPGEILLKVTAAGLCHSDIFVMDMPAAQYAYGLPLTLGHEGVGTVAELGEGVTGFGV
GDAVAVYGPWGCGACHACARGRENYCTRAADLGITPPGLGSPGSMAEYMIVDSARHLVPIGDLDPVAAAPLTDAGLTPYH
AISRVLPLLGPGSTAVVIGVGGLGHVGIQILRAVSAARVIAVDLDDDRLALAREVGADAAVKSGAGAADAIRELTGGQGA
TAVFDFVGAQSTIDTAQQVVAVDGHISVVGIHAGAHAKVGFFMIPFGASVVTPYWGTRSELMEVVALARAGRLDIHTETF
TLDEGPAAYRRLREGSIRGRGVVVP