Crystal structure of human phosphodiesterase 4D2 catalytic domain with inhibitor NPD-1439

4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

hPDE4D2 structure with inhibitor NPD-1439

cAMP-specific 3',5'-cyclic phosphodiesterase 4D

GSHMIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYH
ADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHL
AVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMV
HCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQD
ILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTL

24% PEG 3350, 30% Ethylene Glycol, 0.1 M HEPES

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U values refined individually

phosphodiesterase, hydrolase, cAMP hydrolysis, alternative splicing

IPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVA
YHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGF
KLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCAD
LSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDT
LEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTL