The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa in complex with Bulgecin A

Exolytic and endolytic turnover of peptidoglycan by lytic transglycosylase Slt ofPseudomonas aeruginosa.

QRRLYDQAKAALAKGNSAPYMASRSALRDYPLEPYLAYDELTHRLKSASNEEVERFLTEHGDLPQIGWLKLRWLRLLADR
GDWKTFVNYYDPKLNFTELDCLYGQYQLGHGQKAEGYATSERLWLVGKSQPAACDTLFGLWQGEGQLTEEKVWKRLKLAA
EARNYSLASHLAQRLPTLGNQGALMVSVAQNPAQLSQTGRFSQRDHATADVVGLGLRRLARQDPEKALSLLDYYSSALPF
SSDEKVAIAREIGLSLAKRFDPRALPLMTQYDPGLRDNTVTEWRTRLLLRLGRWDEAYALTRKLPQDLAATSRWRYWQAR
SLQLAQPNSKEPIALYQKLAGERDFYGFLAADRLSVPYKLGNRPAHIDPRVLQRVRNAASTRRAMEFFNRGEVINARREW
YHAARLFDRDELIAQARLAYDMQWYFPAIRSISQAQYWDDLDIRFPMAHRATLVREAKNRGLHSSWIFAITRQQSAFMSD
ARSGVGATGLMQLMPGTAKETSRKFGIPLASTQQLIVPDVNIRLGAAYLSQVHSQFNGNRVLASAAYNAGPGRVRQWLKD
TRHLAFDVWIETIPFDETRQYVQNVLSYAVIYGQKLNAPQPIVDWHERYFDDF

80mM Tris pH 8.5, 12% PEG 8000 and 160mM calcium acetate

National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

QRRLYDQAKAALAKGNSAPYMASRSALRDYPLEPYLAYDELTHRLKSASNEEVERFLTEHGDLPQIGWLKLRWLRLLADR
GDWKTFVNYYDPKLNFTELDCLYGQYQLGHGQKAEGYATSERLWLVGKSQPAACDTLFGLWQGEGQLTEEKVWKRLKLAA
EARNYSLASHLAQRLPTLGNQGALMVSVAQNPAQLSQTGRFSQRDHATADVVGLGLRRLARQDPEKALSLLDYYSSALPF
SSDEKVAIAREIGLSLAKRFDPRALPLMTQYDPGLRDNTVTEWRTRLLLRLGRWDEAYALTRKLPQDLAATSRWRYWQAR
SLQLAQPNSKEPIALYQKLAGERDFYGFLAADRLSVPYKLGNRPAHIDPRVLQRVRNAASTRRAMEFFNRGEVINARREW
YHAARLFDRDELIAQARLAYDMQWYFPAIRSISQAQYWDDLDIRFPMAHRATLVREAKNRGLHSSWIFAITRQESAFMSD
ARSGVGATGLMQLMPGTAKETSRKFGIPLASTQQLIVPDVNIRLGAAYLSQVHSQFNGNRVLASAAYNAGPGRVRQWLKD
TRHLAFDVWIETIPFDETRQYVQNVLSYAVIYGQKLNAPQPIVDWHERYFDDF