Crystal structure of the eIF4E-eIF4G complex from Chaetomium thermophilum in the cap-bound state

Structural motifs in eIF4G and 4E-BPs modulate their binding to eIF4E to regulate translation initiation in yeast.

Eukaryotic translation initiation factor 4E-like protein,Eukaryotic translation initiation factor 4E-like protein

Eukaryotic translation initiation factor 4G

GPHMTVFHDKENFNVKHPLSCRWTLWFTKPASGKGDNWNDLLKKVITFESVEEFWGIYNNIAPVSELAVKSDYHLFKEGV
RPEWEDPQNKHGGKWAYQFKDKRSVNIDELWLHTMLAAIGETLEDEEDGEVMGVVVNVRKGFYRIGVWTRTTEKSKEILM
NIGRRLKEVLKLPPNEMVEFSGHTEAAQAGSTRAKARMVV

GPHMQPSAALQSLRSARFLPGIVQDIYPPGIKSPNPALNEAVQKKGRIFKYDVQFLLQFQNVFTEKPSPDFDQQVKALIG
D

Chaetomium thermophilum var. thermophilum DSM 1495

10% w/v PEG 20000
20% PEG 500 MME
0.02 M 1,6-hexanediol
0.02 M 1-butanol
0.02 M (RS)-1,2-propanediol
0.02 M 2-propanol
0.02 M 1,4-butanediol
0.02 M 1,3-propanediol
0.1 M Bicine/Trizma pH 8.5
0.687 mM m7GpppG

Translation Translational control Translation initiation Gene expression, TRANSLATION

TVFHDKENFNVKHPLSCRWTLWFTKPASGKGDNWNDLLKKVITFESVEEFWGIYNNIAPVSELAVKSDYHLFKEGVRPEW
EDPQNKHGGKWAYQFKDKRSVNIDELWLHTMLAAIGETLEDEEDGEVMGVVVNVRKGFYRIGVWTRTT