Crystal structure of cytochrome P450 CYP260A1 (S276I) bound with progesterone

Structure-Based Engineering of Steroidogenic CYP260A1 for Stereo- and Regioselective Hydroxylation of Progesterone.

MDFPLANLFFVPSEDATAFGRRLRAAAQQAPIVFDTAFGMPILLRKSHITTAYRDTATFSTRMFQAGILNGGLAAMQGDE
HARMRRVYNMFFLPRAVSQYEERFVRPISEQVVDRLAGKPRVDLLEDFAMELPRRVIGELFGFPAEKLHETDERVRAMLR
GLVRMHDPAAVAESQRAYGETLGLITEVVERESRDTSDTLLGEILRTLKAEHMDTIEASRQIVLSLILGGYETTSWLVAN
TIHALLAHPDTLARVRQDPSLLPAAIEEGMRWCPSIFGVLRMVERDVRLDDQALSAGTVVCLAGIAGNYDETAYPSPEVY
DIDRKPLPAANVFGGGAHFCVGAPLARMEARVGLQALLARFPGLRAVPEERPSFMYGAKDSVAHGPDKLPVLLHHHHHHH

0.1 M Bis-Tris propane pH 6.5, 19% (w/v) PEG 3350, 0.05 M MgCl2

Bacterial Proteins, Sorangium cellulosum, Cytochrome P-450 Enzyme System, Cytochrome P450, Hydroxylation, Heme, Oxidation-Reduction, Progesterone, Histidine, Biocatalysis, OXIDOREDUCTASE

MDFPLANLFFVPSEDATAFGRRLRAAAQQAPIVFDTAFGMPILLRKSHITTAYRDTATFSTRMFQAGILNGGLAAMQGDE
HARMRRVYNMFFLPRAVSQYEERFVRPISEQVVDRLAGKPRVDLLEDFAMELPRRVIGELFGFPAEKLHETDERVRAMLR
GLVRMHDPAAVAESQRAYGETLGLITEVVERESRDTSDTLLGEILRTLKAEHMDTIEASRQIVLSLILGGYETTSWLVAN
TIHALLAHPDTLARVRQDPSLLPAAIEEGMRWCPSSFGVLRMVERDVRLDDQALSAGTVVCLAGIAGNYDETAYPSPEVY
DIDRKPLPAANVFGGGAHFCVGAPLARMEARVGLQALLARFPGLRAVPEERPSFMYGAKDSVAHGPDKLPVLLH