NMR solution structure of the cellulose-binding family 2 carbohydrate binding domain (CBM2) from ScLPMO10C
The carbohydrate-binding module and linker of a modular lytic polysaccharide monooxygenase promote localized cellulose oxidation.
Chemical shift assignments for the apo-form of the catalytic domain, the linker region, and the carbohydrate-binding domain of the cellulose-active lytic polysaccharide monooxygenase ScLPMO10C.
Putative secreted cellulose binding protein
HTGSCMAVYSVENSWSGGFQGSVEVMNHGTEPLNGWAVQWQPGGGTTLGGVWNGSLTSGSDGTVTVRNVDHNRVVPPDGS VTFGFTATSTGNDFPVDSIGCVAP
25 mM sodium phosphate, 25 mM sodium chloride, 0.5 mM [U-98% 15N] ScCBM2, 90% H2O/10% D2O
25 mM sodium phosphate, 25 mM sodium chloride, 0.5 mM [U-98% 13C; U-98% 15N] ScCBM2, 100% D2O
25 mM sodium phosphate, 25 mM sodium chloride, 0.5 mM [U-98% 13C; U-98% 15N] ScCBM2, 90% H2O/10% D2O
25 mM sodium phosphate, 25 mM sodium chloride, 0.5 mM ScCBM2, 100% D2O
Orekhov, Jaravine, Mayzel and Kazimierczuk
cellulose binding, beta-sandwich fold, lytic polysaccharide monooxgynease, lpmo, CARBOHYDRATE