Coxsackievirus A24v in complex with the D1-D2 fragment of ICAM-1

Role of enhanced receptor engagement in the evolution of a pandemic acute hemorrhagic conjunctivitis virus.

TBS buffer (Coxsackievirus A24v)
Phosphate buffer (ICAM-1 D1-D2)

Coxsackievirus A24v in complex with the D1 and D2 domains of ICAM-1

Lacey grids coated in a 3 nm carbon film (Agar Scientific, UK)

On-grid binding of the receptor was performed by applying 3 microliters of ICAM-1 (9.85 mg/ml) to the pre-blotted, virus-containing grid, and leaving for 30 seconds before blotting and freezing

GIEETIDTVITNALQLSQPKPQKQPTAQSTPLTSGVNSQEVPALTAVETGASGQAVPSDVIETRHVVNYKTRSESTLESF
FGRSACVTILEVENFNATTDADRKKQFTTWAITYTDTVQLRRKLEFFTYSRFDLEMTFVITERYYASNTGHARNQVYQLM
YIPPGAPRPTAWDDYTWQSSSNPSVFYTYGSAPPRMSIPYVGIANAYSHFYDGFARVPLKDETVDSGDTYYGLVTINDFG
TLAVRVVNEYNPARITSKIRVYMKPKHVRCWCPRPPRAVPYRGEGVDFKQDSITPLTAVENINTF

SPNVEACGYSDRVRQITLGNSTITTQEAANAVVAYGEWPSYLDDKEANPIDAPTEPDVSSNRFYTLDSVQWKSTSRGWWW
KLPDALKDMGMFGQNMYYHYLGRSGYTVHVQCNASKFHQGALGVFAIPEYVMACNTEAKTSYVSYVNANPGEKGGVFDNA
YNPSAEASEGRKFAALDYLLGCGVLAGNAFVYPHQIINLRTNNSATLVLPYVNSLAIDCMAKHNNWGLVILPLCKLDYAP
NSSTEIPITVTIAPMFTEFNGLRNITVPATQ

GLPTMLTPGSSQFLTSDDFQSPCALPNFDVTPPIHIPGEVFNMMELAEIDSMIPMNSVTGKANTMEMYPIPLDDKGSATP
IFSISLSPASDKRLQYTMLGEILNYYTHWTGSLRFTFLFCGSMMATGKILLSYSPPGAKPPTTRKDAMLGTHIIWDLGLQ
SSCTMLAPWISNTVYRRCIKDDFTEGGYITCFYQTRIVVPSGTPTSMFMLAFVSACPDFSVRLLRDTNHISQRTLFARAQ

QTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFL
TVYWT

Netherlands Organization for Scientific Research

VP1, Genome polyprotein, Intercellular adhesion molecule 1

Enterovirus, Receptor, Complex, picornavirus, VIRUS