Crystal structure of the double mutant L167W / P172L of the beta-glucosidase from Trichoderma harzianum

An engineered GH1 beta-glucosidase displays enhanced glucose tolerance and increased sugar release from lignocellulosic materials.

HMLPKDFQWGFATAAYQIEGAIDKDGRGPSIWDTFCAIPGKIADGTSGVTACDSYNRTAEDIALLKSLGAKSYRFSISWS
RIIPKGGRDDPVNQLGIDHYAQFVDDLLEAGITPFITLFHWDLPEELHQRYGGLLNRTEFPLDFENYARVMFKALPKVRN
WITFNEPWCSAILGYGSGTFAPGRQSTTEPWIVGHNLLVAHGRAVKVYRDEFKDLNDGQIGIVLNGDFTYPWDSSDPLDR
EAAERRLEFFTAWYADPIYLGDYPASMRKQLGDRLPEFTPEEKAFVLGSNDFYGMNHYTSNYIRHRTSPATADDTVGNVD
VLFYNKEGQCIGPETESSWLRPCPAGFRDFLVWISKRYNYPKIYVTENGTSLKGENDLPKEKILEDDFRVNYYNEYIRAM
FTAATLDGVNVKGYFAWSLMDNFEWADGYVTRFGVTYVDYENGQQRFPKKSAKSLKPLFDELIAKE

PEG 3350 (20% w/v); 0.2 M sodium nitrate; 0.1 M Bis-tris propane pH 6.5

MLPKDFQWGFATAAYQIEGAIDKDGRGPSIWDTFCAIPGKIADGTSGVTACDSYNRTAEDIALLKSLGAKSYRFSISWSR
IIPKGGRDDPVNQLGIDHYAQFVDDLLEAGITPFITLFHWDLPEELHQRYGGLLNRTEFPLDFENYARVMFKALPKVRNW
ITFNEPLCSAIPGYGSGTFAPGRQSTTEPWIVGHNLLVAHGRAVKVYRDEFKDLNDGQIGIVLNGDFTYPWDSSDPLDRE
AAERRLEFFTAWYADPIYLGDYPASMRKQLGDRLPEFTPEEKAFVLGSNDFYGMNHYTSNYIRHRTSPATADDTVGNVDV
LFYNKEGQCIGPETESSWLRPCPAGFRDFLVWISKRYNYPKIYVTENGTSLKGENDLPKEKILEDDFRVNYYNEYIRAMF
TAATLDGVNVKGYFAWSLMDNFEWADGYVTRFGVTYVDYENGQQRFPKKSAKSLKPLFDELIAKE