OhrA (Organic Hydroperoxide Resistance protein) wild type from chromobacterium violaceum

Substrate and product-assisted catalysis: molecular aspects behind structural switches along Organic Hydroperoxide Resistance Protein catalytic cycle

Organic hydroperoxide resistance protein

MGSSHHHHHHSSGLVPRGSHMNPLQKVLYTAEATATGGRDGRAESSDGALQVKLSTPRELGGLGGDGTNPEQLFAAGYSA
CFIGALKVAAQQAGVRLPAEVSVTGKVSIGPIAHGFGIAAKLAVSLPGLERDAGLRLIEAAHGICPYSNATRGNIEVELT
LA

Purified OhrA protein 10mg/ml in 5mM Tris-Hcl pH 7.4

Crystallisation conditions: Potassium Sodium Tartrate 200mM, Tri-Sodium Citrate 100mM pH 5.5, Ammonium Sulfate 2M

Brazilian National Council for Scientific and Technological Development (CNPq)

The protein could only be found dimerized

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

MNPLQKVLYTAEATATGGRDGRAESSDGALQVKLSTPRELGGLGGDGTNPEQLFAAGYSACFIGALKVAAQQAGVRLPAE
VSVTGKVSIGPIAHGFGIAAKLAVSLPGLERDAGLRLIEAAHGICPYSNATRGNIEVELTLA