Cryo-EM Structure of Mycobacterium smegmatis C(minus) 50S ribosomal subunit

Zinc depletion induces ribosome hibernation in mycobacteria.

Cryo-EM structure of Mycobacterium smegmatis C (minus) 50S ribosomal subunit
Mycobacterium smegmatis C(minus) 70S ribosome with MPY

Relion used for image processing and Refinement

AAGUGUUUAAGGGCGCAUGGUGGAUGCCUUGGCACUGGGAGCCGAUGAAGGACGUAGGAGGCUGCGAUAAGCCUCGGGGA
GCUGUCAACCGAGCGUUGAUCCGAGGAUGUCCGAAUGGGGAAACCCGGCACGAGUGAUGUCGUGUCACCAGGCGCUGAAU
AUAUAGGCGUCUGGGGGGAACGCGGGGAAGUGAAACAUCUCAGUACCCGUAGGAAGAGAAAACAAAAUGUGAUUCCGUGA
GUAGUGGCGAGCGAAAGCGGAGGAUGGCUAAACCGUAUGCAUGUGAUACCGGGUAGGGGUUGUGUGUGCGGGGUUGUGGG
ACCUAUCUUUCCGGCUCUACCUGGCUGGAGGGCAGUGAGAAAAUGUUGUGGUUAGCGGAAAUGGCUUGGGAUGGCCUGCC
GUAGACGGUGAGAGCCCGGUACGUGAAAACCCGACGUCUGUCUUGAUGGUGUUCCCGAGUAGCAGCGGGCCCGUGGAAUC
UGCUGUGAAUCUGCCGGGACCACCCGGUAAGCCUGAAUACUUCCCAGUGACCGAUAGCGGAUUAGUACCGUGAGGGAAUG
GUGAAAAGUACCCCGGGAGGGGAGUGAAAGAGUACCUGAAACCGUGCGCUUACAAUCCGUCAGAGCCCUCGACGUGUCGU
GGGGUGAUGGCGUGCCUUUUGAAGAAUGAGCCUGCGAGUCAGGGACAUGUCGCGAGGUUAACCCGGGUGGGGUAGCCGCA
GCGAAAGCGAGUCUGAAUAGGGCGUAUCCACACAAGAGUGUGUGGUGUAGUGGUGUGUUCUGGACCCGAAGCGGAGUGAU
CUACCCAUGGCCAGGGUGAAGCGCGGGUAAGACCGCGUGGAGGCCCGAACCCACUUAGGUUGAAGACUGAGGGGAUGAGC
UGUGGGUAGGGGUGAAAGGCCAAUCAAACUCCGUGAUAGCUGGUUCUCCCCGAAAUGCAUUUAGGUGCAGCGUCGCAUGU
UUCUUGCCGGAGGUAGAGCUACUGGAUGGCCGAUGGGCCCCACAGGGUUACUGACGUCAGCCAAACUCCGAAUGCCGGUA
AGUCCAAGAGUGCGGCAGUGAGACGGCGGGGGAUAAGCUCCGUGCGUCGAGAGGGAAACAGCCCAGAUCGCCGGCUAAGG
CCCCUAAGCGUGUGCUAAGUGGAAAAGGAUGUGCAGUCGCGAAGACAACCAGGAGGUUGGCUUAGAAGCAGCCACCCUUG
AAAGAGUGCGUAAUAGCUCACUGGUCAAGUGAUUGUGCGCCGAUAAUGUAGCGGGGCUCAAGCACACCGCCGAAGCCGCG
GCAGCCAACGUGUUGGCUGGGUAGGGGAGCGUCCUGCAUCCGGUGAAGCCGCCGAGUGAUCGAGUGGUGGAGGGUGUGGG
AGUGAGAAUGCAGGCAUGAGUAGCGAUUAGGCAAGUGAGAACCUUGCCCGCCGAAAGACCAAGGGUUCCUGGGCCAGGCC
AGUCCGCCCAGGGUGAGUCGGGACCUAAGGCGAGGCCGACAGGCGUAGUCGAUGGACAACGGGUUGAUAUUCCCGUACCC
GUGUAUGUGCGUCCAUGAUGAAUCAGCGGUACUAACCAUCCAAAACCACCGUGACCGCACCUUUCGGGGUGUGGCGUUGG
UGGGGCUGCAUGGGACCUUCGUUGGUAGUAGUCAAGCGAUGGGGUGACGCAGGAAGGUAGCCGUACCGGUCAGUGGUAAU
ACCGGGGUAAGCCUGUAGGGAGUCAGAUAGGUAAAUCCGUCUGGCAUAUAUCCUGAGAGGUGAUGCAUAGCCGAGUGAGG
CGAAUUCGGUGAUCCUAUGCUGCCGAGAAAAGCCUCUAGCGAGGACAUACACGGCCCGUACCCCAAACCAACACAGGUGG
UCAGGUAGAGAAUACUAAGGCGUACGAGUGAACUAUGGUUAAGGAACUCGGCAAAAUGCCCCCGUAACUUCGGGAGAAGG
GGGACCCACAUGGCGUGUAAGCCUUUACGGCCCAAGCGUGAGUGGGUGGCACAAACCAGUGAGAAGCGACUGUUUACUAA
AAACACAGGUCCGUGCGAAGUCGCAAGACGAUGUAUACGGACUGACGCCUGCCCGGUGCUGGAAGGUUAAGAGGACCCGU
UAACUCCCUUUGGGGGUGAAGCGGAGAAUUUAAGCCCCAGUAAACGGCGGUGGUAACUAUAACCAUCCUAAGGUAGCGAA
AUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAACGACUUCUCAACUGUCUCAACCAUAGACUCGGCGAAAUU
GCACUACGAGUAAAGAUGCUCGUUACGCGCGGCAGGACGAAAAGACCCCGGGACCUUCACUACAACUUGGUAUUGGUGCU
CGAUACGGUUUGUGUAGGAUAGGUGGGAGACUGUGAAGCUCACACGCCAGUGUGGGUGGAGUCGUUGUUGAAAUACCACU
CUGAUCGUAUUGGGCCUCUAACCUCGGACCGUAUAUCCGGUUCAGGGACAGUGCCUGGUGGGUAGUUUAACUGGGGCGGU
UGCCUCCUAAAAUGUAACGGAGGCGCCCAAAGGUUCCCUCAACCUGGACGGCAAUCAGGUGUUGAGUGUAAGUGCACAAG
GGAGCUUGACUGCGAGACGGACAUGUCGAGCAGGGACGAAAGUCGGGACUAGUGAUCCGGCACCUCUGAGUGGAAGGGGU
GUCGCUCAACGGAUAAAAGGUACCCCGGGGAUAACAGGCUGAUCUUCCCCAAGAGUCCAUAUCGACGGGAUGGUUUGGCA
CCUCGAUGUCGGCUCGUCGCAUCCUGGGGCUGGAGCAGGUCCCAAGGGUUGGGCUGUUCGCCCAUUAAAGCGGCACGCGA
GCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCUCUAUCCGCCGCGCGCGUCAGAAGCUUGAGGAAACCUGUCCCUAGUA
CGAGAGGACCGGGACGGACGAACCUCUGGUAUACCAGUUGUCCCACCAGGGGCACGGCUGGAUAGCCACGUUCGGACAGG
AUAACCGCUGAAAGCAUCUAAGCGGGAAACCUCUUCCAAGACCAGGCUUCUCACCCUCUAGGAGGGAUAAGGCCCCCCGC
AGACCACGGGAUUGAUAGACCAGACCUGGAAGCCUAGUAAUAGGUGCAGGGAACUGGCACUAACCGGCCGAAAACUUAC

GUUACGGCGGUCCAUAGCGGCAGGGAAACGCCCGGUCCCAUCCCGAACCCGGAAGCUAAGCCUGCCAGCGCCGAUGAUAC
UACCCAUCCGGGUGGAAAAGUAGGACACCGCCGAACAC

MGIRKYKPTTPGRRGASVSDFAEITRSTPEKSLVRPLHGKGGRNAHGRITTRHKGGGHKRAYRVIDFRRHDKDGVNAKVA
HIEYDPNRTANIALLHYLDGEKRYIIAPQGLKQGDVIESGANADIKPGNNLPLRNIPAGTVIHAVELRPGGGAKLARSAG
VSIQLLGKEGTYAALRMPSGEIRRVDVRCRATVGEVGNAEQSNINWGKAGRMRWKGKRPTVRGVVMNPVDHPHGGGEGKT
SGGRHPVSPWGKPEGRTRKPNKPSDKLIVRRRRTGKKR

MARKGILGTKLGMTQVFDENNKVVPVTVVKAGPNVVTRIRTTERDGYSAVQLAYGEISPRKVIKPVAGQFAAAGVNPRRH
VAELRLDDEAAVAEYEVGQELTAEIFSDGAYVDVTGTSKGKGFAGTMKRHGFRGQGAAHGAQAVHRRPGSIGGCATPGRV
FKGTRMSGRMGNDRVTTQNLKVHKVDAENGVLLIKGAIPGRNGGLVVVRSAIKRGEK

TLKVDVKTPAGKTDGSVELPAELFDVEPNIALMHQVVTAQLAAKRQGTHSTKTRGEVSGGGKKPYRQKGTGRARQGSTRA
PQFTGGGTVHGPKPRDYSQRTPKKMIAAALRGALSDRARNDRIHAVTELVEGQTPSTKSAKTFLGTLTENKKVLVVIGRT
DEVGAKSVRNLPGVHVISPDQLNTYDVLNADDVVFSVEALNAYISANSKEGASV

TTTEKALPRLKQRYREEIREALQQEFNYANVMQIPGVVKVVVNMGVGDAARDAKLINGAINDLALITGQKPEVRRARKSI
AQFKLREGMPIGARVTLRGDRMWEFLDRLISIALPRIRDFRGLSPKQFDGTGNYTFGLNEQSMFHEIDVDSIDRPRGMDI
TVVTTATNDAEGRALLRALGFPFKEN

MSRIGKQPVPVPSGVDVTINGQNLSVKGPKGTLTLDVAEPISVSRAEDGAIVVTRPDDERRSRSLHGLSRTLIANLVTGV
TEGYTQKMEIFGVGYRVQLKGQNLEFALGYSHPVLIEAPEGITFAVESPTKFSVSGIDKQKVGQISAVIRRLRRPDPYKG
KGVRYEGEQIRRKVGKTGK

MKLILTAEVEHLGAAGDTVEVKDGYGRNYLLPRGLAIVASRGAERQAEEIRRARESKVIRDIEHANELKTALEGLGDVTL
SVNAAGDTGKLFGSVTAADVVNAIKKAGGPNLDKRTVQLAKAHIKSVGTHPVTVKLHTGVEAKVSLNVVAQ

MAKADKATAVADIAEQFKASTATVVTEYRGLTVANLAELRRALGDSATYTVAKNTLVKRAASEAGIEGLDELFAGPTAIA
FVKGEAVDAAKAIKKFAKDNKALVIKGGYMDGKALSVADVEKIADLESREVLLAKLAGAMKGNLSKAAGLFNAPASQVAR
LAAALQEKKAGEEAA

MAPKKKVAGLIKLQIQAGQANPAPPVGPALGQHGVNIMEFCKAYNAATESQRGNVIPVEITVYEDRSFTFALKTPPAAKL
LLKAAGVQKGSGEPHKTKVAKVTWDQVREIAETKKADLNANDIDAAAKIIAGTARSMGITVE

PTYTPKAGDTTRSWYVIDASDVVLGRLASAAATLLRGKHKPTFTPNVDGGDFVIVINADKIAVSGDKLTKKFAYRHSGYP
GGLRKRTIGELLEKHPTRVVENAIIGMLPHNKLGRQIQKKLKVYAGPDHPHAAQQPIPFEIKQVAQ

MIQQESRLKVADNTGAKEILCIRVLGGSSRRYAGIGDVIVATVKDAIPGGNVKRGDVVKAVVVRTVKERRRADGSYIKFD
ENAAVIIKNDNDPRGTRIFGPVGRELREKKFMKIVSLAPEVL

MSVIKLHDLKPAPGEKKAKTRVGRGEGSKGKTAGRGTKGTKARKNVPVMFEGGQMPIHMRLPKLKGFKNRFRTEYQVVNV
GDINKAFPQGGTVGVDELVAKGLVRKNSLVKVLGDGKLTVKVDVTANKFSGSAREAITAAGGSATEL

MLIPRKVKHRKQHHPEQRGIASGGTSVSFGDYGIQALEHAYITNRQIESARIAINRHIKRGGKVWINIFPDRPLTKKPAE
TRMGSGKGSPEWWVANVKPGRVLFELSYPDEKTARDALTRAIHKLPIKARIVTREEQF

MPKPTKGPRLGGSSSHQSALLANLATSLFEHGRIKTTEPKARALRPYAEKLITHAKKGALHNRREVMKKIRDKDVVHTLF
AEIGPFYADRNGGYTRIIKVENRKGDNAPMAVIELVREKTVTDEANRARRAAASQAKADERADEKADEKAEETVEETTEA
PAEESTEAAAEETVEETTEAPAEESTEAAEESEAKDDTK

AHKPVGQNISEVRRNARLRRHARLRKKVAGTAEVPRLVVNRSARHIHVQLVNDLNGTTLAAASSIEADVRAIDGDKKAHS
VRVGQLIAERAKAAGVETVVFDRGGYTYGGRIAALADAAREAGLKF

MNTLDFVDQASLRDDIPTFSPGDTVNVHVKVIEGSKERIQVFKGVVIRRQGGGISETFTVRKESYGVGVERTFPVHSPNI
DHIDVLTRGDVRRAKLYYLRELRGKKAKIKEKR

MARVKRALNAQKKRRTVLKASKGYRGQRSRLYRKAKEQQLHSLTYAYRDRRARKGEFRKLWISRINAAARANDITYNRLI
QGLKAAGVEVDRKNLAELAVSDPAAFTALVDVARAALPEDVNAPSGEAA

ATYAIVKTGGKQYKVAAGDVVKVEKLDSEPGASVSLPVALVVDGANVTSKADDLAKVAVTAEVLEHTKGPKIRIHKFKNK
TGYHKRQGHRQQLTVLKVTGIK

STVTEFPSATAKARYVRVSATKARRVIDLVRGKSVEEALDILRWAPQAASEPVAKVIASAAANAQNNEGLDPSTLVVATV
YADEGPTAKRIRPRAQGRAFRIRKRTSHITVIVESRPPKQKGASAASARSRRAQGSKAAATKKSAETKEGSE

ATITDPRDIILAPVISEKSYGLIEDNVYTFVVHPDSNKTQIKIAIEKIFDVKVDSVNTANRQGKRKRTRTGFGKRKSTKR
AIVKLAAGSKPIDLFGAPA

MKVHKGDTVLVISGKDKGAKGKVLVAYPDRNKVLVEGVNRIKKHTAVSANERGASSGGIVTQEAPIHVSNVMVVDSDGKP
TRVGYRIDDETGKKVRIAKTNGKDI

MAKTANIPNKLTANVRTRTGKGASRQARRDGKVPAVLYGHGTDPQHLELNARDFAAVLRSHGTNAILTLDIEGTEQLALT
KALDVHPIRRNIQHADLLVVQRGEKVTVEVTVLVEGDATPGTLVTQDANTIEIEAEALSIPEQLTVSVEGVEAGTQITAG
QISLPEGVNLISDPELLVVNVVEAPSAEALEEEGAGETGAAEAPAEEAPAEEPAE

MAHKKGASSSRNGRDSAAQRLGVKRFGGQVVKAGEILVRQRGTHFHPGVNVGRGGDDTLFALAPGAVEFGAKRGRKTVNI
VPVARPEA

MKPGIHPDYHPVVFQDAATGAQFLTRSTATSTRTIEWPTPSGPKTYPLIVVDVTSDSHPFWTGSARHIDSAGQVEKFRRR
YG

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)