LsrB from Clostridium saccharobutylicum in complex with AI-2

Identification of novel autoinducer-2 receptors inClostridiareveals plasticity in the binding site of the LsrB receptor family.

GSFTSSNTTTDTSTNSSKKGNVTVTFIPKLTGNAFFESANKGAQKYSEQWGFKVDYEGDANASAASQVSVINKAVQQGTN
AICLSSVDAAGVKDALKAAADAGVTVTTWDSDVDPSVRKVMVSQGTPEQLGQMLVQMGYDSLKERGKDPEKDAIKYCWHY
SNATVTDQNSWQVEGEKYIKSKYPNWQNVAPDNYYSNQDAEQAISVGESILSAHSDIDLIICNDSTALPGQAQAAQNKGL
TAKNVTITGFASPNSMKQYCNDGILTRWGLWDCGIQGAMGCYMAYYIASGNSVKVGDKIEIPTVGTVEVMPNSVLDPKAD
DSDTSSGVVLLPERTIFTKDNMNNYDF

0.1 M Citric acid pH 2.75 and 26% w/v PEG 3350

SSNTTTDTSTNSSKKGNVTVTFIPKLTGNAFFESANKGAQKYSEQWGFKVDYEGDANASAASQVSVINKAVQQGTNAICL
SSVDAAGVKDALKAAADAGVTVTTWDSDVDPSVRKVMVSQGTPEQLGQMLVQMGYDSLKERGKDPEKDAIKYCWHYSNAT
VTDQNSWQVEGEKYIKSKYPNWQNVAPDNYYSNQDAEQAISVGESILSAHSDIDLIICNDSTALPGQAQAAQNKGLTAKN
VTITGFASPNSMKQYCNDGILTRWGLWDCGIQGAMGCYMAYYIASGNSVKVGDKIEIPTVGTVEVMPNSVLDPKADDSDT
SSGVVLLPERTIFTKDNMNNYDF