Crystal structure of the C-terminal ARM domain of Homo sapiens SPIN90 (SH3-protein interacting with Nck), residues 351-722

Structure of the nucleation-promoting factor SPIN90 bound to the actin filament nucleator Arp2/3 complex.

54 kDa VacA-interacting protein, 54 kDa vimentin-interacting protein, VIP54, 90 kDa SH3 protein interacting with Nck, AF3p21, Dia-interacting protein 1, DIP-1, Diaphanous protein-interacting protein, SH3 adapter protein SPIN90, WASP-interacting SH3-domain protein, WISH, Wiskott-Aldrich syndrome protein-interacting protein

SPVMEQVLLSLVEGKDLSMALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEGVIRCYLEELLHILTDADPEVC
KKMCKRNEFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDHQKLCYSALILA
MVFSMGEAVPYAHYEHLGTPFAQFLLNIVEDGLPLDTTEQLPDLCVNLLLALNLHLPAADQNVIMAALSKHANVKIFSEK
LLLLLNRGDDPVRIFKHEPQPPHSVLKFLQDVFGSPATAAIFYHTDMMALIDITVRHIADLSPGDKLRMEYLSLMHAIVR
TTPYLQHRHRLPDLQAILRRILNEEETSPQCQMDRMIVREMCKEFLVLGEAPS

N-terminal GST-fusion with TEV cleavage site

50 mM Tris, pH 7.5, 700 mM sodium potassium tartrate

Department of Health & Human Services (HHS)

inactive form, N-terminally truncated SPIN90, 6 and 2-3 armadillo repeats, unable to activate Arp2-3 complex, ENDOCYTOSIS