Trans form of HemolysinII c-terminal domain
GSHMDNQKALEEQMNSINSVNDKLNKGKGKLSLSMNGNQLKATSSNAGYGISYEDKNWGIFVNGEKVYTFNEKSTVGNIS NDINKLNIKGPYIEIKQI
Escherichia coli 'BL21-Gold(DE3)pLysS AG'
structures with the least restraint violations
1.0 mM [U-99% 13C; U-99% 15N] HlyIIC, 20 mM sodium phosphate, 1 mM EDTA, 0.05 % w/v sodium azide, 1 mM AEBSF protease inhibitor, 90% H2O/10% D2O
1.0 mM [U-99% 15N] HlyIIC, 20 mM sodium phosphate, 1 mM EDTA, 1 mM AEBSF protease inhibitor, 0.05 % w/v sodium azide, 90% H2O/10% D2O
1.0 mM [U-99% 15N] HlyIIC, 20 mM sodium phosphate, 1.0 mM EDTA, 1.0 mM AEBSF protease inhibitor, 0.05 % w/v sodium azide, 100% D2O
Schwieters, Kuszewski, Tjandra and Clore
pore-forming toxin, proline isomerization, conformational heterogeneity, TOXIN
DNQKALEEQMNSINSVNDKLNKGKGKLSLSMNGNQLKATSSNAGYGISYEDKNWGIFVNGEKVYTFNEKSTVGNISNDIN KLNIKGPYIEIKQI