The arsenate respiratory reductase (Arr) complex from Shewanella sp. ANA-3 bound to arsenate

2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE

Structural and mechanistic analysis of the arsenate respiratory reductase provides insight into environmental arsenic transformations.

4Fe-4S ferredoxin, iron-sulfur binding domain protein

GAELPAPLRRTGVGEWLATTCQGCTSWCAKQIYVMDGRALKVRGNPNSGVHGMSSCPRQHLSLQQVYDPDRLRTPMMRTN
PKKGRDQDPKFVPISWDKALDMLADKIIALRVANEPHKYALLRGRYSHINDLLYKKMTNLIGSPNNISHSSVCAEAHKMG
PYYLDGNWGYNQYDVKNAKFILSFGADPIASNRQVSFYSQTWGDSLDHAKVVVVDPRLSASAAKAHKWIPIEPGQDSVLA
LAIAHVALVEGVWHKPFVGDFIEGKNLFKAGKTVSVESFKETHTYGLVEWWNQALKDYTPEWASKITGIDPKTIIAIAKD
MGAAAPAVQVWTSRGAVMQARGTYTSISCHALNGLFGGIDSKGGLFPGNKTPLLKEYPEAKAYMDEIAAKGVKKEKIDQR
GRLEFPALAKGKSGGGVITANAANGIRNQDPYEIKVMLAYFNNFNFSNPEGQRWDEALSKVDFMAHITTNVSEFSWFADV
LLPSSHHMFEKWGVLDSIGNGVAQISIQQPSIKRLWDTRIDESEIPYMLAKKLADKGFDAPWRYINEQIVDPETGKPAAD
EAEFAKLMVRYLTAPLWKEDASKYGDKLSSWDEFVQKGVWNSSPYKLEARWGKFKTETTKFEFYSKTLEKALQSHADKHK
VSIDEVMKACDYQARGHLAFIPHYEEPYRFGDESEFPLLLVDQKSRLNKEGRTANSPWYYEFKDVDPGDVANEDVAKFNP
IDGKKFGLKDGDEIRITSPVGMLTCKAKLWEGVRPGTVAKCFGQGHWAYGRYASAKFGVTPRGGSNNDLIADRYDRLSGA
SAFYGHIRVRVEKV

MRLGMVIDLQKCVGCGGCSLACKTENNTNDGIHWSHHIATTEGTFPDVKYTYIPTLCNHCDDAPCVKVCPTGAMHKDKRG
LTLQNNDECIGCKKCMNACPYGVISFNAATPHRRWQDDSEVVANGTVSPLMLLKRTGATATPNENPERGDTYPMIRPKRT
TEKCTFCDHRLDKGLNPACVDACPSEARVIGDLDDPQSKVSQLIKLHKPMQLKPEAGTGPRVFYIRSFGVKTAY

10 mg/ml protein, 15% PEG 2000 MME, 150 mM KSCN, 50 mM HEPES (adjusted to pH 7.5 with NaOH), 5 mM disodium arsenate, microseeded; cryo protected in 30% PEG 2000 MME, 100 mM KSCN, 1 M sodium formate, 50 mM MES (pH 6.0), 5 mM disodium arsenate

National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)

arsenate, molybdoprotein, molybdopterin, OXIDOREDUCTASE

AELPAPLRRTGVGEWLATTCQGCTSWCAKQIYVMDGRALKVRGNPNSGVHGMSSCPRQHLSLQQVYDPDRLRTPMMRTNP
KKGRDQDPKFVPISWDKALDMLADKIIALRVANEPHKYALLRGRYSHINDLLYKKMTNLIGSPNNISHSSVCAEAHKMGP
YYLDGNWGYNQYDVKNAKFILSFGADPIASNRQVSFYSQTWGDSLDHAKVVVVDPRLSASAAKAHKWIPIEPGQDSVLAL
AIAHVALVEGVWHKPFVGDFIEGKNLFKAGKTVSVESFKETHTYGLVEWWNQALKDYTPEWASKITGIDPKTIIAIAKDM
GAAAPAVQVWTSRGAVMQARGTYTSISCHALNGLFGGIDSKGGLFPGNKTPLLKEYPEAKAYMDEIAAKGVKKEKIDQRG
RLEFPALAKGKSGGGVITANAANGIRNQDPYEIKVMLAYFNNFNFSNPEGQRWDEALSKVDFMAHITTNVSEFSWFADVL
LPSSHHMFEKWGVLDSIGNGVAQISIQQPSIKRLWDTRIDESEIPYMLAKKLADKGFDAPWRYINEQIVDPETGKPAADE
AEFAKLMVRYLTAPLWKEDASKYGDKLSSWDEFVQKGVWNSSPYKLEARWGKFKTETTKFEFYSKTLEKALQSHADKHKV
SIDEVMKACDYQARGHLAFIPHYEEPYRFGDESEFPLLLVDQKSRLNKEGRTANSPWYYEFKDVDPGDVANEDVAKFNPI
DGKKFGLKDGDEIRITSPVGMLTCKAKLWEGVRPGTVAKCFGQGHWAYGRYASAKFGVTPRGGSNNDLIADRYDRLSGAS
AFYGHIRVRVEKV