Crystal structure of zebrafish Phosphatidylinositol-4-phosphate 5- kinase alpha isoform with bound ATP/Ca2+

Structural insights into lethal contractural syndrome type 3 (LCCS3) caused by a missense mutation of PIP5K gamma.

Phosphatidylinositol-4-phosphate 5-kinase, type I, alpha

Phosphatidylinositol-4-phosphate 5-kinase,type I,alpha,a

MASTGETTYKKTTSSALKGAIQLGITHSVGSLSQKPERDVLMQDFEVVESIFFPSQGSSSTPGHHHGDFKFKTYAPIAFR
YFREMFGIRPDDYLYSLCNEPLIELSNPGASGSLFYVSSDDEFIIKTVQHKEAEFLQTLLPGYFMNLNQNMRTLLPKFYG
LYCVQADGKNIRIVVMNNLLPRAVPMHLKFDLKGSTYKRRASPKERSKGVPTYKDLDFMQDMPEGILLENDHYTALSRTM
QRDCRVLQSFKIMDYSLLVGIHILHRAGEEASTAVPDTQKKGQGQKPLYCTAIESIQGESKSKTSPQPYESMGGIPAFNS
KGERLLVFIGIIDILQSYRLVKKLEHSWKALLHDGDTVSVHRPSFYADRFQKFMCSTVFRKSQLKTLEHHHHHH

10 mg/ml protein with 2 mM ATP and 10 mM MgCl2, mixed with 14% PEG8000, 100 mM sodium cacodylate, pH 6.5, 160 mM calcium acetate and 20% glycerol

TGETTYKKTTSSALKGAIQLGITHSVGSLSQKPERDVLMQDFEVVESIFFPSQGSSSTPGHHHGDFKFKTYAPIAFRYFR
EMFGIRPDDYLYSLCNEPLIELSNPGASGSLFYVSSDDEFIIKTVQHKEAEFLQTLLPGYFMNLNQNMRTLLPKFYGLYC
VQADGKNIRIVVMNNLLPRAVPMHLKFDLKGSTYKRRASPKERSKGVPTYKDLDFMQDMPEGILLENDHYTALSRTMQRD
CRVLQSFKIMDYSLLVGIHILHRAGEEASTAVPDTQKKGQGQKPLYCTAIESIQGESKSKTSPQPYESMGGIPAFNSKGE
RLLVFIGIIDILQSYRLVKKLEHSWKALLHDGDTVSVHRPSFYADRFQKFMCSTVFRKSQLKT