Crystal Structure of CDC45 from Entamoeba histolytica

Crystal Structure ofEntamoeba histolyticaCdc45 Suggests a Conformational Switch that May Regulate DNA Replication.

Cell division control protein 45 cdc45 putative

(MSE)IINGTSFEELYNGLRNRGKDKKTIQLYVSPTVDAIVCYKILS(MSE)(MSE)FEKDGLLHSAVPVNNYETLSRVF
KETIGHTDVHTVFFIDCAGSIDVSELLGDIENIFVYIIDSHRPFNKLNVTNTNIGLITSNEYQQFIEQETQLEKSDLLEV
DDDLIGLNKSQSFNNCEYIRK(MSE)VDSDGEFYSESVGRVALAIAKKLNKVEND(MSE)YWYAAIGVCDQYISLKINAK
TYVHAIQYFIDNLQLETLEITDLLQTVKTP(MSE)CVK(MSE)DCQL(MSE)LLRHWTLYDSLFHTREIASKLGIWTSRG
KEKFDVLIAD(MSE)GIPLSQAQQSYKT(MSE)SLE(MSE)KNKFLLK(MSE)EGYSKFYHFENLFLPSFFKKFG(MSE)
DYSISAFDAAHAIGSIITNDEPDQNWQQQFWEGFKLLSSTTAEPYDFGFAKCIESNKNLVETGIILLLSGSCFNEANKYR
FCSVSDELLSIRFKTPYKALQLAQFLAEASSRRYKKWLPFILAVLDAEKKTFVIVGYSSPISVKTLNYFGAKFTQTAQN
(MSE)KISILQKSFDSFVTEIHRENLVKYKKALHK(CME)FTSI

MIINGTSFEELYNGLRNRGKDKKTIQLYVSPTVDAIVCYKILSMMFEKDGLLHSAVPVNNYETLSRVFKETIGHTDVHTV
FFIDCAGSIDVSELLGDIENIFVYIIDSHRPFNKLNVTNTNIGLITSNEYQQFIEQETQLEKSDLLEVDDDLIGLNKSQS
FNNCEYIRKMVDSDGEFYSESVGRVALAIAKKLNKVENDMYWYAAIGVCDQYISLKINAKTYVHAIQYFIDNLQLETLEI
TDLLQTVKTPMCVKMDCQLMLLRHWTLYDSLFHTREIASKLGIWTSRGKEKFDVLIADMGIPLSQAQQSYKTMSLEMKNK
FLLKMEGYSKFYHFENLFLPSFFKKFGMDYSISAFDAAHAIGSIITNDEPDQNWQQQFWEGFKLLSSTTAEPYDFGFAKC
IESNKNLVETGIILLLSGSCFNEANKYRFCSVSDELLSIRFKTPYKALQLAQFLAEASSRRYKKWLPFILAVLDAEKKTF
VIVGYSSPISVKTLNYFGAKFTQTAQNMKISILQKSFDSFVTEIHRENLVKYKKALHKCFTSI

sodium thiocyanate (pH 6.9), polyethyleneglycol 3,350

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Cell division control protein 45 CDC45, putative

Cell division control protein, CELL CYCLE