Crystal structure of Transient Receptor Potential (TRP) channel TRPV4 in the presence of gadolinium

Cryo-EM and X-ray structures of TRPV4 reveal insight into ion permeation and gating mechanisms.

Transient receptor potential cation channel, subfamily V, member 4

MAPSPDPPPVIKMFNRHMLFDIVSRGSTAELEGFLPFLLAQKKRLTDEEFREASTGKTCLTKALMNLNGGKNDTIPMLID
IAEKTGNLREFINSPFRDVYYRGQTALHIAIERRCKHYVELLVEKGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACT
NQPDIVHYLTENAHKKADIRRQDSRGNTVLHALVAIADNTRENTKFVTKVYDLLVIKCVKLYPDSSLEAIFNNDSMSPLM
MAAKLGKIGIFQHIIRLEIKDEEARHLSRKFRDWAYGPVYSSLYDLSMLDTCGEEVSVLEILVYNSKVENRHEMLAVEPI
NELLRDKWQKFGAVSFYISVVSYLIAMIIFTLIAYYRPMDGTPPYPYRTTMDYMRLAGEIVTLLTGVVFFITNIKDLFMK
KCPGVNSLFIDGSFQLLYFIYSVLVIITAVLYLVGIESYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMLQKILFKDLF
RFLLVYLLFMIGYASALVSLLNPCTSQESCIETSSQCTVPEYPSCRDSSTFSKFLLDLFKLTIGMGDLEMINSAKYPAVF
IILLVTYIILTFVLLLNMLIALMGETVGQVSKESKQIWKLQWATTILDIERSFPVCMRKAFRSGEMVTVGKNLDGTPDRR
WCFRVDEVNWSHWNQNLGIINEDPGRSNSLEVLFQ

50 mM MES pH6.0, 100 mM NaCl, 5.5% PEG4000, and 10% glycerol

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

APSPDPPPVIKMFNRHMLFDIVSRGSTAELEGFLPFLLAQKKRLTDEEFREASTGKTCLTKALMNLNGGKNDTIPMLIDI
AEKTGNLREFINSPFRDVYYRGQTALHIAIERRCKHYVELLVEKGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTN
QPDIVHYLTENAHKKADIRRQDSRGNTVLHALVAIADNTRENTKFVTKVYDLLVIKCVKLYPDSSLEAIFNNDSMSPLMM
AAKLGKIGIFQHIIRLEIKDEEARHLSRKFRDWAYGPVYSSLYDLSMLDTCGEEVSVLEILVYNSKVENRHEMLAVEPIN
ELLRDKWQKFGAVSFYISVVSYLIAMIIFTLIAYYRPMDGTPPYPYRTTMDYMRLAGEIVTLLTGVVFFITNIKDLFMKK
CPGVNSLFIDGSFQLLYFIYSVLVIITAVLYLVGIESYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMLQKILFKDLFR
FLLVYLLFMIGYASALVSLLNPCTSQESCIETSSNCTVPEYPSCRDSSTFSKFLLDLFKLTIGMGDLEMINSAKYPAVFI
ILLVTYIILTFVLLLNMLIALMGETVGQVSKESKQIWKLQWATTILDIERSFPVCMRKAFRSGEMVTVGKNLDGTPDRRW
CFRVDEVNWSHWNQNLGIINEDPGR