PLP-dependent L-arginine hydroxylase RohP quinonoid I complex

(2E)-5-carbamimidamido-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}pentanoic acid

Snapshots of the Catalytic Cycle of an O2, Pyridoxal Phosphate-Dependent Hydroxylase.

MGSSHHHHHHSSGLVPRGSHMHPQATPAPGAPLLDLTQHEIQALTMKYNLADAHTHQRQSASQQSIVSRLPQLWYEAEEG
LQATYEKRFTEAFFQLHRQPTALVKNKTMLSYAASISTMVAGMFLKKERLAVTLIEPCFDNLYDVLANMDVPLYPIDESV
FYDVDRIYPELERRVRTDALFLVDPNNPTGFSLLRHGRKGFEEVVRFCKDHDKLLLIDFCFASFTLFEPELARFDMYELL
ENSGVRYLAIEDTGKTWPVQDAKCALITASDDIWETVYNLHTSVLLNVSPFVLNMLTQYVRDSAADRLASVREVLTRNRE
CARKTLDGSILEYQEPVVKVSVAWFRVDHPELTATDVHRLLSADGVYVLPGRYFYWSEPSKGDAYVRMALAREPEMFADA
MALTRQVLDRHGR

0.2 M sodium malonate pH 7, 16-20% (w/v) PEG 3350

Natural Sciences and Engineering Research Council (NSERC, Canada)

O2-, PLP-dependent hydroxylase, BIOSYNTHETIC PROTEIN