Actinin-1 EF-Hand bound to the Cav1.2 IQ Motif
Structural Basis of the Localization and Activation of Neuronal L-type Ca2+ Channels by a-Actinin1 and Calmodulin
Chemical shift assignments of the C-terminal EF-hand domain of a-actinin-1.
Voltage-dependent L-type calcium channel subunit alpha-1C
Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1
Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, Voltage-gated calcium channel subunit alpha Cav1.2
Escherichia coli 'BL21-Gold(DE3)pLysS AG'
National Institutes of Health/National Eye Institute (NIH/NEI)
All samples were performed with the following buffer system: 20 mM Tris-d11 (pH 7.5) with 1 mM DTT d10, 1 mM EDTA-d12 and 95 % H2O/5 % D2O
also distance geometry and torsion angle dynamics
300 uM [U-99% 15N] Actinin-1 EF hand, 300 uM IQ Motif, 90% H2O/10% D2O
15N labeled Actinin-1 EF hand 3,4 with unlabeled IQ Motif
15N labeled Cav1.2 IQ Motif bound to unlabeled actinin-1 EF3,4
Double Labeled Actinin-1 EF 3,4 bound to IQ Motif
12 mg/mL Pf1 phage, 300 uM [U-99% 15N] Actinin-1 EF 3,4, 300 uM Cav1.2 IQ Motif, 90% H2O/10% D2O
Samples created with 12 mg/mL Pf1 phage for RDC calculation
12 mg/mL Pf1 phage, 300 uM [U-99% 15N] Cav1.2 IQ Motif, 300 uM Actinin-1 EF 3,4, 90% H2O/10% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Markus Zweckstetter and Ad Bax J. Am. Chem. Soc. , 122, (2000) 3791-3792 Contact: zweckste@speck.niddk.nih.gov
Light Scatter was performed as well as Fluorescent polarization to confirm binding.
actinin-1, EF hand, Calcium Calmodulin, Neuronal signaling, Voltage Gated Calcium Channel, Cav1.2, Voltage Gated Calcium Channel 1.2, Hippocampal Neurons, IQ Motif, SIGNALING PROTEIN