GFGNFGTS from low-complexity/FG repeat domain of Nup98, residues 116-123
Atomic structures of low-complexity protein segments reveal kinked beta sheets that assemble networks.
Density map was obtained using measured diffraction intensities and the phases acquired from a crystallographic direct methods program, SHELXD.
The peptide was solubilized by adding nano-pure H2O with 20% DMSO to achieve a concentration of 12 mg/mL. The peptide solution was immediately used for crystallization. Crystals grew at room temperature by hanging drop vapor diffusion.
Phase statistics are not applicable. No imaging was used. The phases wwere obtained by a crystallographic direct methods program, SHELXD.
A crystal containing protofilaments of an 8-residue segment of Nup98
The detector was operated in rolling shutter mode with 2x2 pixel binning.
GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
Nuclear pore complex protein Nup98-Nup96
National Institutes of Health/Office of the Director
National Science Foundation (NSF, United States)
Amyloid, LARKS, Reversible-amyloid, low-complexity, FG repeat, PROTEIN FIBRIL