Two-State 14-mer UUCG Tetraloop calculated from Exact NOEs (State one: Conformers 1-5, State Two: Conformers 6-10)
High-resolution small RNA structures from exact nuclear Overhauser enhancement measurements without additional restraints.
RNA (5'-R(P*GP*GP*CP*AP*CP*UP*UP*CP*GP*GP*UP*GP*CP*C)-3')
University of Colorado Anschutz Medical Campus
2D WaterGate [1H-1H] NOESY buildup series
20 mM KHPO4, 0.4 mM EDTA, 1 mM 14-mer UUCG Tetraloop, 95% H2O/5% D2O
20 mM KHPO4, 0.4 mM EDTA, 1.7 mM 14-mer UUCG Tetraloop, 100% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
5 mm triple resonance 1H/13C/15N cold probe with z-axis gradient
RNA, Exact NOE, UUCG tetraloop, 14-mer hairpin, Two-State