Crystal structure of V278E-glyoxalase I mutant from Zea mays in space group P4(1)2(1)2

Deciphering the number and location of active sites in the monomeric glyoxalase I of Zea mays.

convex prefocussing mirror and a Kirkpatrick-Baez pair of focussing mirrors

cryogenically cooled channel cut crystal monochromator

GSHMASMATGSEASKAAEAVVDWHKHDSKRMLHAVYRVGDLDRTIKYYTECFGMKLLRKRDVPDEKYTNAFLGFGPENTN
FAVELTYNYGVDKYDIGTGFGHFAIANDDVYKLAENIKSKGGKITREPGPVKGGSTVIAFAQDPDGYMFELIQRADTPEP
LCQVMLRVGDLERSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVLELTYNYGVTEYSKGNAYAQVAIGTNDV
YKSAEAVDLATKELGGKILRQPGPLPGINTKIASFVDPDGWKVELVDNTDFLKELH

sodium formate 4.2 M pH 9.7 0.5% PEG 4000

MATGSEASKAAEAVVDWHKHDSKRMLHAVYRVGDLDRTIKYYTECFGMKLLRKRDVPDEKYTNAFLGFGPENTNFAVELT
YNYGVDKYDIGTGFGHFAIANDDVYKLAENIKSKGGKITREPGPVKGGSTVIAFAQDPDGYMFELIQRADTPEPLCQVML
RVGDLERSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVLELTYNYGVTEYSKGNAYAQVAIGTNDVYKSAEA
VDLATKELGGKILRQPGPLPGINTKIASFVDPDGWKVVLVDNTDFLKELH