Crystal structure of Acetyl-CoA acetyltransferase from Elizabethkingia anophelis NUHP1

Seattle Structural Genomics Center for Infectious Disease (SSGCID)

MAHHHHHHMKEVFIVSAVRTPMGSFMGSLSGVPATQLGAVAIKGALDKINLNPAEIQDVYMGNVLQAGEGQAPAKQAALG
AGLPNTTPTTAVNKV(SCY)ASGMKAVMMAAQAVKAGDVEAIVAGGMENMSQVPHYIDGRNGVKLGDIKLQDGLLKDGLT
DVYSKQHMGN(SCY)AELCAKEYNITREEQDAFAIQSYERSAKAWSEGKFKEEVVPVSIPQRKGEPIIFAEDEEYKNVKF
DRIPTLPTVFQKENGTVTAANASTLNDGASALVLMSKEKMESLGLKPLAKIVSYADAAQAPEWFTTAPAKALPIALAKAN
LTINDIDFFEFNEAFSVVGLANNKILGLDAAKVNVNGGAVALGHPLGSSGSRIIVTLINVLKQNNAKYGAAAICNGGGGA
SAIVIENIK

MAHHHHHHMKEVFIVSAVRTPMGSFMGSLSGVPATQLGAVAIKGALDKINLNPAEIQDVYMGNVLQAGEGQAPAKQAALG
AGLPNTTPTTAVNKVCASGMKAVMMAAQAVKAGDVEAIVAGGMENMSQVPHYIDGRNGVKLGDIKLQDGLLKDGLTDVYS
KQHMGNCAELCAKEYNITREEQDAFAIQSYERSAKAWSEGKFKEEVVPVSIPQRKGEPIIFAEDEEYKNVKFDRIPTLPT
VFQKENGTVTAANASTLNDGASALVLMSKEKMESLGLKPLAKIVSYADAAQAPEWFTTAPAKALPIALAKANLTINDIDF
FEFNEAFSVVGLANNKILGLDAAKVNVNGGAVALGHPLGSSGSRIIVTLINVLKQNNAKYGAAAICNGGGGASAIVIENI
K

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

18.9 mg/mL ElanA.00654.a.B1.PS38241 + 3 mM AcetylCoA + RigakuReagents JCSG+ screen, E11: 14.5% w/v PEG8000, 20% w/v glycerol, 160 mM calcium acetate, 100 mM cacodylate/HCl, pH 6.5, cryoprotectant: direct, tray 291032e11, puck DYA4-6

Seattle Structural Genomics Center for Infectious Disease
NIAID, National Institute of Allergy and Infectious Diseases

SSGCID, Structural Genomics, Elizabethkingia anophelis, Acetyl-CoA acetyltransferase, Seattle Structural Genomics Center for Infectious Disease, TRANSFERASE

MKEVFIVSAVRTPMGSFMGSLSGVPATQLGAVAIKGALDKINLNPAEIQDVYMGNVLQAGEGQAPAKQAALGAGLPNTTP
TTAVNKVCASGMKAVMMAAQAVKAGDVEAIVAGGMENMSQVPHYIDGRNGVKLGDIKLQDGLLKDGLTDVYSKQHMGNCA
ELCAKEYNITREEQDAFAIQSYERSAKAWSEGKFKEEVVPVSIPQRKGEPIIFAEDEEYKNVKFDRIPTLPTVFQKENGT
VTAANASTLNDGASALVLMSKEKMESLGLKPLAKIVSYADAAQAPEWFTTAPAKALPIALAKANLTINDIDFFEFNEAFS
VVGLANNKILGLDAAKVNVNGGAVALGHPLGSSGSRIIVTLINVLKQNNAKYGAAAICNGGGGASAIVIENI