The NZ-1 Fab complexed with the PDZ tandem fragment of A. aeolicus S2P homolog with the PA12 tag inserted between the residues 263 and 267

Application of the NZ-1 Fab as a crystallization chaperone for PA tag-inserted target proteins.

PDZ tandem fragment with PA tag insertion

Light chain of antigen binding fragment, Fab of NZ-1

Heavy chain of antigen binding fragment, Fab of NZ-1

GSEVPKYLKEPVVVGYVQRDSIAQKIGIKPGDKIIKI(SNN)GYEVRTWEDLRDALIRLSLDGVKETTLFLERNGEVLHL
TIKVPNVQKGEELGIAPLVKPVVGGVKKGSPADQVGIKPGDLILEVNGKKINTWYELVEEVRKSQGKAIKLKILRGVAMP
GAEDDVVMIEKELIPAKDPKTGTYFIGLFPKTE

GSEVPKYLKEPVVVGYVQRDSIAQKIGIKPGDKIIKINGYEVRTWEDLRDALIRLSLDGVKETTLFLERNGEVLHLTIKV
PNVQKGEELGIAPLVKPVVGGVKKGSPADQVGIKPGDLILEVNGKKINTWYELVEEVRKSQGKAIKLKILRGVAMPGAED
DVVMIEKELIPAKDPKTGTYFIGLFPKTE

(PCA)FVLTQPNSVSTNLGSTVKLSCKRSTGNIGSNYVNWYQQHEGRSPTTMIYRDDKRPDGVPDRFSGSIDRSSNSALL
TINNVQTEDEADYFCHSYSSGIVFGGGTKLTVLGQPKSTPTLTVFPPSTEELQGNKATLVCLISDFYPSDVEVAWKANGA
PISQGVDTANPTKQGNKYIASSFLRLTAEQWRSRNSFTCQVTHEGNTVEKSLSPAECV

QFVLTQPNSVSTNLGSTVKLSCKRSTGNIGSNYVNWYQQHEGRSPTTMIYRDDKRPDGVPDRFSGSIDRSSNSALLTINN
VQTEDEADYFCHSYSSGIVFGGGTKLTVLGQPKSTPTLTVFPPSTEELQGNKATLVCLISDFYPSDVEVAWKANGAPISQ
GVDTANPTKQGNKYIASSFLRLTAEQWRSRNSFTCQVTHEGNTVEKSLSPAECV

EVQLVESGGGLVQPGRSLKLSCAASGFTFSNYGMAWVRQTPTKGLEWIASISAGGDKTYYGDSVKGRFSISRDNAKTTHY
LQMDSLRSEDTATYYCAKTSRVYFDYWGQGVMVTVSSAETTAPSVYPLAPGTALKSNSMVTLGCLVKGYFPEPVTVTWNS
GALSSGVHTFPAVLQSGLYTLTSSVTVPSSTWSSQAVTCNVAHPASSTKVDKKIVPREC

15-20% (wt./vol.) PEG 3350, 200mM ammonium citrate (pH 7.0)

Japan Society for the Promotion of Science

Japan Agency for Medical Research and Development (AMED)

Authors state that three residues of the host protein, that is 264, 265, and 266, were deleted and 12 residues, GVAMPGAEDDVV, were inserted in place of them.

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

EVPKYLKEPVVVGYVQRDSIAQKIGIKPGDKIIKINGYEVRTWEDLRDALIRLSLDGVKETTLFLERNGEVLHLTIKVPN
VQKGEELGIAPLVKPVVGGVKKGSPADQVGIKPGDLILEVNGKKINTWYELVEEVRKSQGKAIKLKILR