Crystal structure of mouse claudin-3 P134A mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin

1-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Morphologic determinant of tight junctions revealed by claudin-3 structures.

GHMASGSMSMGLEITGTSLAVLGWLCTIVCCALPMWRVSAFIGSSIITAQITWEGLWMNCVVQSTGQMQCKMYDSLLALP
QDLQAARALIVVSILLAAFGLLVALVGAQATNAVQDETAKAKITIVAGVLFLLAALLTLVAVSWSANTIIRDFYNPLVPE
AQKREMGAGLYVGWAAAALQLLGGALLAAS

GSAAATERLNLTDALNSNPAGNLYDWRSSNSYPWTQKLNLHLTITATGQKYRILASKIVDFNIYSNNFNNLVKLEQSLGD
GVKDHYVDISLDAGQYVLVMKANSSYSGNYPYAILFQKF

HEPES, Sodium acetate, Magnesium nitrate, PEG 3350

AUTHORS STATE THAT THE N-TERMINAL RESIDUES GHMASGS IN A AND C CHAINS ARE DERIVED FROM THE TEV PROTEASE CLEAVAGE SITE AND LINKER AND THAT GLY201 AND SER202 IN B AND D CHAINS ARE DERIVED FROM THE THROMBIN CLEAVAGE SITE.

Cell adhesion, Tight junction, MEMBRANE PROTEIN-TOXIN complex

MSMGLEITGTSLAVLGWLCTIVCCALPMWRVSAFIGSSIITAQITWEGLWMNCVVQSTGQMQCKMYDSLLALPQDLQAAR
ALIVVSILLAAFGLLVALVGAQCTNCVQDETAKAKITIVAGVLFLLAALLTLVPVSWSANTIIRDFYNPLVPEAQKREMG
AGLYVGWAAAALQLLGGALLCCS

AAATERLNLTDALNSNPAGNLYDWRSSNSYPWTQKLNLHLTITATGQKYRILASKIVDFNIYSNNFNNLVKLEQSLGDGV
KDHYVDISLDAGQYVLVMKANSSYSGNYPYSILFQKF