Crystal structure of endo-arabinanase ABN-TS D27A mutant in complex with arabinotriose

alpha-L-arabinose; L-arabinose; arabinose

Structures of endo-1,5-alpha-L-arabinanase mutants from Bacillus thermodenitrificans TS-3 in complex with arabino-oligosaccharides.

MVHFHPFGNVNFYEMDWSLKGDLWAHAPVIAKEGSRWYVFHTGSGIQIKTSEDGVHWENMGRVFPSLPDWCKQYVPEKDE
DHLWAPDICFYNGIYYLYYSVSTFGKNTSVIGLATNRTLDPRDPDYEWKDMGPVIHSTASDNYNAIDPNVVFDQEGQPWL
SFGSFWSGIQLIQLDTETMKPAAQAELLTIASRGEEPNAIEAPFIVCRNGYYYLFVSFDFCCRGIESTYKIAVGRSKDIT
GPYVDKNGVSMMQGGGTILDAGNDRWIGPGHCAVYFSGVSAILVNHAYDALKNGEPTLQIRPLYWDDEGWPYLLEHHHHH
H

20% PEG 8000, 0.2M magnesium chloride, 0.1M Tris-HCl pH 8.5

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

arabinanase, thermostable enzyme, glycoside hydrolase, gh43, HYDROLASE

MVHFHPFGNVNFYEMDWSLKGDLWAHDPVIAKEGSRWYVFHTGSGIQIKTSEDGVHWENMGRVFPSLPDWCKQYVPEKDE
DHLWAPDICFYNGIYYLYYSVSTFGKNTSVIGLATNRTLDPRDPDYEWKDMGPVIHSTASDNYNAIDPNVVFDQEGQPWL
SFGSFWSGIQLIQLDTETMKPAAQAELLTIASRGEEPNAIEAPFIVCRNGYYYLFVSFDFCCRGIESTYKIAVGRSKDIT
GPYVDKNGVSMMQGGGTILDAGNDRWIGPGHCAVYFSGVSAILVNHAYDALKNGEPTLQIRPLYWDDEGWPYL