Crystal structure of the modified fructosyl peptide oxidase from Aspergillus nidulans with 7 additional mutations, in complex with FSA

1-S-(carboxymethyl)-1-thio-beta-D-fructose; 1-S-(carboxymethyl)-1-thio-D-fructose; 1-S-(carboxymethyl)-1-thio-fructose

Creation of haemoglobin A1c direct oxidase from fructosyl peptide oxidase by combined structure-based site specific mutagenesis and random mutagenesis.

MAPRANTKIIVVGGGGTMGSSTALHLLRAGYTPSNITVLDTYPIPSAQSAGYDLNKIFGISGANKPDLQLSLEAFDMWKN
DPLFKPFFHNVGQMDVSSTEEGIKKLRKRYQSLLRAGIGLEKTNFLLESEDEILAKAPHFTREQIKGWKGLFCGDGGWLA
AAKAINAIGQFLKEQGVKFGFGEAGTFKKPLFADADEKTCIGVETVDGTKYYADKVVLAAGAWSSTLVDLEEQCVSKAWV
FAHIQLTPAEAAAYKNTPVIYDGDYGFFIEPDENGIIKVCDEFPGFTHFKMHQPYGSPVPKLISVPRSHAKHPTDTYPHA
SEVTIKKAINRFLPRFNDKELFNRAMCWCTDTADANLLVCEHPRWKGFYLATGDSGHSFKLLPNIGKHVVELLEGRLESV
FKDAWRWRPGSGDALKSRRAAPAKDLADMPGWRNEAKM

fructosyl peptide, oxidoreductase, Aspergillus nidulans, FAD-binding protein