Crystal structure of the modified fructosyl peptide oxidase from Aspergillus nidulans with R61G mutation, in complex with FSA

1-S-(carboxymethyl)-1-thio-beta-D-fructose; 1-S-(carboxymethyl)-1-thio-D-fructose; 1-S-(carboxymethyl)-1-thio-fructose

Creation of haemoglobin A1c direct oxidase from fructosyl peptide oxidase by combined structure-based site specific mutagenesis and random mutagenesis.

MAPRANTKIIVVGGGGTMGSSTALHLLRAGYTPSNITVLDTYPIPSAQSAGYDLNKIFGIGLRNKPDLQLYLEALDMWKN
DPLFKPFFHNVGQMDVSSTEEGIKKLRMRYQSLLDAGIGLEKTNFLLESEDEILAKAPHFTREQIKGWKGLF(CSO)GDG
GWLAAAKAINAIGQFLKEQGVKFGFGEAGTFKKPLFADADEKTCIGVETVDGTKYYADKVVLAAGAWSSTLVDLEEQCVS
KAWVFAHIQLTPAEAAAYKNTPVIYDGDYGFFIEPDENGIIKVCDEFPGFTHFKMHQPYGSPVPKLISVPRSHAKHPTDT
YPHASEVTIKKAINRFLPRFNDKELFNRAMCWCTDTADANLLVCEHPRWKGFYLATGDSGHSFKLLPNIGKHVVELLEGR
LESVFKDAWRWRPGSGDALKSRRAAPAKDLADMPGWRNEAKM

MAPRANTKIIVVGGGGTMGSSTALHLLRAGYTPSNITVLDTYPIPSAQSAGYDLNKIFGIGLRNKPDLQLYLEALDMWKN
DPLFKPFFHNVGQMDVSSTEEGIKKLRMRYQSLLDAGIGLEKTNFLLESEDEILAKAPHFTREQIKGWKGLFCGDGGWLA
AAKAINAIGQFLKEQGVKFGFGEAGTFKKPLFADADEKTCIGVETVDGTKYYADKVVLAAGAWSSTLVDLEEQCVSKAWV
FAHIQLTPAEAAAYKNTPVIYDGDYGFFIEPDENGIIKVCDEFPGFTHFKMHQPYGSPVPKLISVPRSHAKHPTDTYPHA
SEVTIKKAINRFLPRFNDKELFNRAMCWCTDTADANLLVCEHPRWKGFYLATGDSGHSFKLLPNIGKHVVELLEGRLESV
FKDAWRWRPGSGDALKSRRAAPAKDLADMPGWRNEAKM

1.75 M ammonium sulfate, 0.2 M lithium sulfate, 0.08 M CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer (pH 10.4)

fructosyl peptide, oxidoreductase, Aspergillus nidulans, FAD-binding protein