Crystal structure of a cis-epoxysuccinate hydrolase producing D(-)-tartaric acids

2-Gaussian fit: Grosse-Kunstleve RW, Sauter NK, Adams PD: Newsletter of the IUCr Commission on Crystallographic Computing 2004, 3, 22-31.

Structural insight into the catalytic mechanism of a cis-epoxysuccinate hydrolase producing enantiomerically pure d(-)-tartaric acid.

MGSSHHHHHHSSGLVPRGSHMTRTKLILEARINEYMPRRGNPHVPWTPKEIGEAAAQAREAGASIVHFHARQADGSPSHD
YETYAESIREIRARSDVLVHPTLGQITLGGRESRLAHIERLCLDPALKPDFAPVALGSTNIDRYDDVEKRYETGDRVYLN
NIDTLQHFSKRLRELGVKPAFIAWTVPFTRTLDAFMDMGLVDDPAYLLFELTDCGIRGGHPGTIRGLRAHTDFLPPGRQI
QWTVCNKIGNLFGPAAAAIEEGGHVAIGLGDYLYPELGTPTNGEVVQTVANMARAMGREIATPAETKEILGISN

0.1 M Tris pH 8.5, 0.1 M magnesium formate dihydrate

National Natural Science Foundation of China

chain 'A' and (resid 23 through 130 )
chain 'A' and (resid 131 through 197 )
chain 'A' and (resid 198 through 312 )
chain 'B' and (resid 23 through 80 )
chain 'B' and (resid 81 through 130 )
chain 'B' and (resid 131 through 150 )
chain 'B' and (resid 151 through 175 )
chain 'B' and (resid 176 through 239 )
chain 'B' and (resid 240 through 281 )
chain 'B' and (resid 282 through 295 )
chain 'B' and (resid 296 through 312 )
chain 'C' and (resid 23 through 61 )
chain 'C' and (resid 62 through 130 )
chain 'C' and (resid 131 through 175 )
chain 'C' and (resid 176 through 281 )
chain 'C' and (resid 282 through 296 )
chain 'C' and (resid 297 through 312 )
chain 'D' and (resid 23 through 130 )
chain 'D' and (resid 131 through 261 )
chain 'D' and (resid 262 through 312 )
chain 'E' and (resid 23 through 123 )
chain 'E' and (resid 124 through 140 )
chain 'E' and (resid 141 through 161 )
chain 'E' and (resid 162 through 175 )
chain 'E' and (resid 176 through 197 )
chain 'E' and (resid 198 through 261 )
chain 'E' and (resid 262 through 281 )
chain 'E' and (resid 282 through 295 )
chain 'E' and (resid 296 through 312 )
chain 'F' and (resid 23 through 130 )
chain 'F' and (resid 131 through 261 )
chain 'F' and (resid 262 through 312 )
chain 'G' and (resid 23 through 130 )
chain 'G' and (resid 131 through 261 )
chain 'G' and (resid 262 through 312 )
chain 'H' and (resid 23 through 115 )
chain 'H' and (resid 116 through 130 )
chain 'H' and (resid 131 through 150 )
chain 'H' and (resid 151 through 175 )
chain 'H' and (resid 176 through 197 )
chain 'H' and (resid 198 through 261 )
chain 'H' and (resid 262 through 281 )
chain 'H' and (resid 282 through 295 )
chain 'H' and (resid 296 through 312 )

MTRTKLILEARINEYMPRRGNPHVPWTPKEIGEAAAQAREAGASIVHFHARQADGSPSHDYETYAESIREIRARSDVLVH
PTLGQITLGGRESRLAHIERLCLDPALKPDFAPVDLGSTNIDRYDDVEKRYETGDRVYLNNIDTLQHFSKRLRELGVKPA
FIAWTVPFTRTLDAFMDMGLVDDPAYLLFELTDCGIRGGHPGTIRGLRAHTDFLPPGRQIQWTVCNKIGNLFGPAAAAIE
EGGHVAIGLGDYLYPELGTPTNGEVVQTVANMARAMGREIATPAETKEILGISN