Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide

beta-D-glucuronic acid; D-glucuronic acid; glucuronic acid

Dissection of the substrate preference and structure of gut microbial-glucuronidases identifies the major bacteria causing xenobiotic toxicity

Glycosyl hydrolase family 2, TIM barrel domain protein

MHHHHHHSSGVDLGTENLYFQSNGMLYPQSNDSRIVFPLDGVWDFRTAGEDSYPAEWADAPLPEPLPMAVPGSYNDQNDE
LNLRAHYGWVVYQRSFAVPSRLVAGQRMILRFDAATHAADVYLNGQLLGSHFGGFLPFEFDVTSALHAGENLLTVAVDNR
IGSSTLPVGNDAGTAFMGSDNANVPAVAEAKKHARRQNLPNFDFFNFAGLNRHVELYTTPADAYIADIAITTERLDHIAG
DACTAANALIAYDVTFGGDFPSNPTDPNTLIQPSDPAINHADPSESSESDIQRTATYGRQVRISILDGEGTVVAGVTADI
ERSGDGTAKASGEIAIRDAKLWNPGAAYLYTAVAELLPEGGAESSSRIIDAYRQTFGIRTVEVSGTTFLINGKPFYFKGF
GKHEDSYFHGRGTDDVLNVKDVSLIHWLHANSFRTSHYPYAESMYDLCDREGIVIIDEVPAVGMSWLQYANPLVAERHRE
AIRGMIARDKNHPCIVMWSIANAPGLDGDGERPRQAYDYFRPLYELAHASDPQNRPVTLVCCQNDYTTDITERTMDVVCI
NRYYGWYNLSGDLDAACHALNIELDFWENIGKPVMFTEYGADTIEGIHGTHGEMFSEEFQRDYYARINAEIDKRPWFIGE
QLWNFADFATFQGIIRVEGNRKGILTRDRQPKMAAHWLRERWAGIPDYGYKG

100mM Sodium cacodylate, pH 6.5, 18%(w/v) PEG2000 MME

MLYPQSNDSRIVFPLDGVWDFRTAGEDSYPAEWADAPLPEPLPMAVPGSYNDQNDELNLRAHYGWVVYQRSFAVPSRLVA
GQRMILRFDAATHAADVYLNGQLLGSHFGGFLPFEFDVTSALHAGENLLTVAVDNRIGSSTLPVGNDAGTAFMGSDNANV
PAVAEAKKHARRQNLPNFDFFNFAGLNRHVELYTTPADAYIADIAITTERLDHIAGDACTAANALIAYDVTFGGDFPSNP
TDPNTLIQPSDPAINHADPSESSESDIQRTATYGRQVRISILDGEGTVVAGVTADIERSGDGTAKASGEIAIRDAKLWNP
GAAYLYTAVAELLPEGGAESSSRIIDAYRQTFGIRTVEVSGTTFLINGKPFYFKGFGKHEDSYFHGRGTDDVLNVKDVSL
IHWLHANSFRTSHYPYAESMYDLCDREGIVIIDEVPAVGMSWLQYANPLVAERHREAIRGMIARDKNHPCIVMWSIANEP
GLDGDGERPRQAYDYFRPLYELAHASDPQNRPVTLVCCQNDYTTDITERTMDVVCINRYYGWYNLSGDLDAACHALNIEL
DFWENIGKPVMFTEYGADTIEGIHGTHGEMFSEEFQRDYYARINAEIDKRPWFIGEQLWNFADFATFQGIIRVEGNRKGI
LTRDRQPKMAAHWLRERWAGIPDYGYKG