The crystal structure of human IYD Thr239 mutant with ligand 3-Fluorotyrosine (F-Tyr)

Redox control of iodotyrosine deiodinase

The role of Thr239 on the catalysis of hIYD

FMN(flavin mononucleotide)
YOF(3-monofluorotyrosine)

GEPRTRAEARPWVDEDLKDSSDLHQAEEDADEWQESEENVEHIPFSHNHYPEKEMVKRSQEFYELLNKRRSVRFISNEQV
PMEVIDNVIRTAGTAPSGAHTEPWTFVVVKDPDVKHKIRKIIEEEEEINYMKRMGHRWVTDLKKLRTNWIKEYLDTAPIL
ILIFKQVHGFAANGKKKVHYYNEISVSIACGILLAALQNAGLVTVTTAPLNCGPRLRVLLGRPAHEKLLMLLPVGYPSKE
ATVPDLKRKPLDQIMVTVHHHHHH

0.17 M sodium acetate, 85 mM Tris-HCl (pH 8.5), 22.5 % w/v polyethylene glycol 4,000 and 15 % glycerol.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

hIYD, FMN, MFT, T239A mutant, OXIDOREDUCTASE

GEPRTRAEARPWVDEDLKDSSDLHQAEEDADEWQESEENVEHIPFSHNHYPEKEMVKRSQEFYELLNKRRSVRFISNEQV
PMEVIDNVIRTAGTAPSGAHTEPWTFVVVKDPDVKHKIRKIIEEEEEINYMKRMGHRWVTDLKKLRTNWIKEYLDTAPIL
ILIFKQVHGFAANGKKKVHYYNEISVSIACGILLAALQNAGLVTVTTTPLNCGPRLRVLLGRPAHEKLLMLLPVGYPSKE
ATVPDLKRKPLDQIMVTV