Crystal structure of Isosesquilavandulyl Diphosphate Synthase from Streptomyces sp. strain CNH-189 in complex with GPP

"Head-to-Middle" and "Head-to-Tail" cis-Prenyl Transferases: Structure of Isosesquilavandulyl Diphosphate Synthase.

MAHHHHHHVDDDDKMMTNLMLLPDGMRRWSQKQGISLDDSYAAMTDKLVEFTGWAREEGFTTFYVTVSSVANYSRSEEQV
TTAMNAFTEVVRRCHDTLNFNYSGTLEVVPERWLTELEALRAKSDSQSDFTLHFIMGMSLAHEVIGIFNKFNGKIPALTE
ELLAANAYVPEPVDFLIRPGGHVRMSSFYPLMSPFAEMYFCPTLLNDMTRADFDVALEDLRERDRRYGLYPV

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

PROTEIN: 10 mg/mL in 25 mM Tris-Cl (pH 7.5) and 150 mM NaCl
RESERVOIR: 0.2 M (NH4)2SO4, 0.1 M MES pH 6.5, 30% PEG 5000MME
SOAK: 5 mM MgCl2, 5 mM (NH4)4P2O7, 0.1 M MES pH 6.5, 30% PEG 5000MME, 10 mM GPP

prenyltransferase, antibiotic biosynthesis, TRANSFERASE

MTNLMLLPDGMRRWSQKQGISLDDSYAAMTDKLVEFTGWAREEGFTTFYVTVSSVANYSRSEEQVTTAMNAFTEVVRRCH
DTLNFNYSGTLEVVPERWLTELEALRAKSDSQSDFTLHFIMGMSLAHEVIGIFNKFNGKIPALTEELLAANAYVPEPVDF
LIRPGGHVRMSSFYPLMSPFAEMYFCPTLLNDMTRADFDVALEDLRERDRRYGLYPV