Aspergillus kawachii beta-fructofuranosidase

Crystal structure of a beta-fructofuranosidase with high transfructosylation activity from Aspergillus kawachii

MSVVIDYNVAPPNLSTLPNGSLFETWRPRAHVLPPNGQIGDPCLHYTDPATGLFHVGFLHDGSGISSATTDDLATYQDLN
QGNQVIVPGGINDPVAVFDGSVIPNGINGLPTLLYTSVSYLPIHWSIPYTRGSETQSLAVSSDGGSNFTKLDQGPVIPGP
PFAYNVTAFRDPYVFQNPTLDSLLHSKNNTWYTVISGGLHEKGPAQFLYRQYDSDFQYWEYLGQWWHEPTNSTWGNGTWA
GRWAFNFETGNVFSLDEYGYNPHGQIFTTIGTEGSDLPVVPQLTSIHDMLWVSGTVSRNGSVSFNPNMAGFLDWGFSSYA
AAGKVLPSTSLPSTKSGAPDRFISYVWLSGDLFEQAEGFPTNQQNWTGTLLLPRELRVLYIPNVVDNALARESGASWQVV
SSDGSAGTVELQTLGISIARETKAALLSGTSFTESGRTLNSSGVVPFKRSPSEKFFVLSAQLSFPASARGSGLKSGFQIL
SSEHESTTVYYQFSNESIIVDRSNTSAAARTTDGIDSSAEAGKLRLFDVLNGGEQAIETLDLTLVVDNSVLEVYANGRFA
LSTWVRSWYANSTNISFFHNGVGGVAFSKVTVSEGLYDAWPDRQY

0.1 M sodium phosphate, 2.6 M sodium formate

The same proetein complexed with glycerol

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

fructooligosaccharides, 1-kestose, GH32, beta-propeller, HYDROLASE

SVVIDYNVAPPNLSTLPNGSLFETWRPRAHVLPPNGQIGDPCLHYTDPATGLFHVGFLHDGSGISSATTDDLATYQDLNQ
GNQVIVPGGINDPVAVFDGSVIPNGINGLPTLLYTSVSYLPIHWSIPYTRGSETQSLAVSSDGGSNFTKLDQGPVIPGPP
FAYNVTAFRDPYVFQNPTLDSLLHSKNNTWYTVISGGLHEKGPAQFLYRQYDSDFQYWEYLGQWWHEPTNSTWGNGTWAG
RWAFNFETGNVFSLDEYGYNPHGQIFTTIGTEGSDLPVVPQLTSIHDMLWVSGTVSRNGSVSFNPNMAGFLDWGFSSYAA
AGKVLPSTSLPSTKSGAPDRFISYVWLSGDLFEQAEGFPTNQQNWTGTLLLPRELRVLYIPNVVDNALARESGASWQVVS
SDGSAGTVELQTLGISIARETKAALLSGTSFTESGRTLNSSGVVPFKRSPSEKFFVLSAQLSFPASARGSGLKSGFQILS
SEHESTTVYYQFSNESIIVDRSNTSAAARTTDGIDSSAEAGKLRLFDVLNGGEQAIETLDLTLVVDNSVLEVYANGRFAL
STWVRSWYANSTNISFFHNGVGGVAFSKVTVSEGLYDAWPDRQY