11S globulin from Wrightia tinctoria reveals auxin binding site

A novel function for globulin in sequestering plant hormone: Crystal structure of Wrightia tinctoria 11S globulin in complex with auxin.

MASTSFLQVLIALICLVLLCERSCLASRQSSPQSITEKQGIGECDLQRVNPLEPAHRIQHEAGYSEIWDPTSRELQCAGI
DATRHVIENRGLFVPSYNNAPMLIIVVQGHGILGAVFPGCPETFQSFHPTEKEGGDSRGPDQTFRDQHQKVHFIRQGDVI
ALPAGIVHWAYNEATEKLVLLVIHDLSNRENQLDQNLRRYFLGGNQKGDEETWAVGSKNLLWNNVFQPLDPQFLGRASGV
NSEIIKKLQSENDFRGYMVRVRDGLRLVRPSSEEPRPPRPLLDNGYEETLCTVRIKENLLNPERADIYTSRGGTVSTLNS
YNLPILRKLQLSANREYLYPNAMIVPEWNNNAHSISYVTRGSGRLQVGGSSKSTVYDGDVRQGQLFIIPQNYVYLKQAGP
QGLELYTVKTNDRAKATALVGRTSVIRAVPLDVWINVFQLTQDEARSLKYNREEITVLDPELPKPQPEGGGYYPWSIVA

0.2 M Sodium citrate tribasic dihydrate, 100 mM Tris hydrochloride pH 8.5, 30% v/v PEG 400

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

MASTSFLQVLIALICLVLLCERSCLASRQSSPQSITEKQGIGECDLQRVNPLEPAHRIQHEAGYSEIWDPTSRELQCAGI
DATRHVIENRGLFVPSYNNAPMLIIVVQGHGILGAVFPGCPETFQSFHPTEKEGGDSRGPDQTFRDRHQKVHFIRQGDVI
ALPAGIVHWAYNEATEKLVLLVIHDLSNRENQLDQNLRRYFLGGNQKGDEETWAVGSKNLLWNNVFQPLDPQFLGRASGV
NSEIIKKLQSENDFRGYMVRVRDGLRLVRPSSEEPRPPRPLLDNGYEETLCTVRIKENLLNPERADIYTSRGGTVSTLNS
YNLPILRKLQLSANREYLYPNAMIVPEWNNNAHSISYVTRGSGRLQVGGSSKSTVYDGDVRQGQLFIIPQNYVYLKQAGP
QGLELYTVKTNDRAKATALVGRTSVIRAVPLDVWINVFQLTQDEARSLKYNREEITVLGPELPKPQPEGGGYYPWSIVA