Crystal Structure Analysis of primosome protein DnaB (resiues 1-300) from Geobacillus stearothermophilus

Structural analyses of the bacterial primosomal protein DnaB reveal that it is a tetramer and forms a complex with a primosomal re-initiation protein

Replication initiation and membrane attachment protein

MQHHWKELIAVDRYTVQSRGVLQEVDRKVLTLLYQPLIGCRALALYMTLWGELELLDGQEATHHRLMALMQCGLPDIYSE
RLKLEGIGLLDTYVHAKEADEPKLFLYELRPPLAPDQFFRDEMLSVFLRRQVGRHLFIQLSNFFARPSIDETKFTQVTRS
FSDVFSAVPAEQIVAGLSEEAGPELLEGKDHIRRDEASYVLDDGVFDFELFFAGLSKQLVPRRAVTAKVKEAIKKLAFLY
GIPPLEMQKLVLGVIDPAYHIDIDALRRAAREWYELEHGGVEPRLVERVQPLAYRTMENI

0.1M Tris (pH 8.5), 1.0M Ammonium sulfate

Primosome, DnaB, DNA replication, REPLICATION