Cryo-EM structure of B. subtilis flagellar filaments H84R

A structural model of flagellar filament switching across multiple bacterial species.

Images were stored containing seven parts, where each part represented a set of frames corresponding to a dose of ~20 electrons per Angstrom^2. The full dose image stack was used for the estimation of the CTF as well as for boxing filaments. Only the first two parts were used for the reconstruction (~5 electrons per Angstrom^2).

MRINHNIAALNTLNRLSSNNSASQKNMEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLEMASKNSQDGISLIQTAEGAL
TETRAILQRVRELVVQAGNTGTQDKATDLQSIQDEISALTDEIDGISNRTEFNGKKLLDGTYKVDTATPANQKNLVFQIG
ANATQQISVNIEDMGADALGIKEADGSIAALHSVNDLDVTKFADNAADCADIGFDAQLKVVDEAINQVSSQRAKLGAVQN
RLEHTINNLSASGENLTAAESRIRDVDMAKEMSEFTKNNILSQASQAMLAQANQQPQNVLQLLR

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

Cryo-EM structure of B. subtilis flagellar filaments H84R'

helical filament was observed by negative staining and Cryo-EM
bacteria flagella, helical polymers, cryo-EM, PROTEIN FIBRIL

MRINHNIAALNTLNRLSSNNSASQKNMEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLEMASKNSQDGISLIQTAEGAL
TETHAILQRVRELVVQAGNTGTQDKATDLQSIQDEISALTDEIDGISNRTEFNGKKLLDGTYKVDTATPANQKNLVFQIG
ANATQQISVNIEDMGADALGIKEADGSIAALHSVNDLDVTKFADNAADTADIGFDAQLKVVDEAINQVSSQRAKLGAVQN
RLEHTINNLSASGENLTAAESRIRDVDMAKEMSEFTKNNILSQASQAMLAQANQQPQNVLQLLR