Cryo-EM structure of B. subtilis flagellar filaments A39V, N133H

A structural model of flagellar filament switching across multiple bacterial species.

Images were stored containing seven parts, where each part represented a set of frames corresponding to a dose of ~20 electrons per Angstrom^2. The full dose image stack was used for the estimation of the CTF as well as for boxing filaments. Only the first two parts were used for the reconstruction (~5 electrons per Angstrom^2).

MRINHNIAALNTLNRLSSNNSASQKNMEKLSSGLRINRVGDDAAGLAISEKMRGQIRGLEMASKNSQDGISLIQTAEGAL
TETHAILQRVRELVVQAGNTGTQDKATDLQSIQDEISALTDEIDGISNRTEFHGKKLLDGTYKVDTATPANQKNLVFQIG
ANATQQISVNIEDMGADALGIKEADGSIAALHSVNDLDVTKFADNAADCADIGFDAQLKVVDEAINQVSSQRAKLGAVQN
RLEHTINNLSASGENLTAAESRIRDVDMAKEMSEFTKNNILSQASQAMLAQANQQPQNVLQLLR

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

helical filament was observed by negative staining and Cryo-EM
A helical assembly can be generated by applying the helical parameters to a single subunit, e.g., chain A.
bacteria flagella, helical polymers, cryo-EM, PROTEIN FIBRIL

MRINHNIAALNTLNRLSSNNSASQKNMEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLEMASKNSQDGISLIQTAEGAL
TETHAILQRVRELVVQAGNTGTQDKATDLQSIQDEISALTDEIDGISNRTEFNGKKLLDGTYKVDTATPANQKNLVFQIG
ANATQQISVNIEDMGADALGIKEADGSIAALHSVNDLDVTKFADNAADTADIGFDAQLKVVDEAINQVSSQRAKLGAVQN
RLEHTINNLSASGENLTAAESRIRDVDMAKEMSEFTKNNILSQASQAMLAQANQQPQNVLQLLR