Crystal structure of the influenza virus PA endonuclease in complex with inhibitor 10k (SRI-30027)

Protein-Structure Assisted Optimization of 4,5-Dihydroxypyrimidine-6-Carboxamide Inhibitors of Influenza Virus Endonuclease.

Loop 51-72 is replaced with a GGS linker, N-Terminal has His-tag residues

MGSSHHHHHHSSGLVPRGSHMEDFVRQCFNPMIVELAEKAMKEYGEDPKIETNKFAAICTHLEVCFMYSDGGSKHRFEII
EGRDRIMAWTVVNSICNTTGVEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSFTGEEMATKA
DYTLDEESRARIKTRLFTIRQEMASRSLWDSFRQSER

0.1 M CAPSO pH 9.5, 1 M Ammonium Sulfate, 10 mM MnCl2, 10 mM MgCl2

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

St. Jude Children's Research Hospital (ALSAC)

Virus, Nuclease, Transcription, Cap-snatching, HYDROLASE, HYDROLASE-HYDROLASE INHIBITOR complex

KHRFEIIEGRDRIMAWTVVNSICNTTGVEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSFTG
EEMATKADYTLDEESRARIKTRLFTIRQEMASRSLWDSFRQSER