Crystal structure of the influenza virus PA endonuclease in complex with inhibitor 6d (SRI-29680)

5-hydroxy-6-oxo-2-[(2S)-1-(phenoxyacetyl)pyrrolidin-2-yl]-1,6-dihydropyrimidine-4-carboxylic acid

Protein-Structure Assisted Optimization of 4,5-Dihydroxypyrimidine-6-Carboxamide Inhibitors of Influenza Virus Endonuclease.

Loop 51-72 replaced with a GGS linker, C-Terminal has residual His-tag residues

MEDFVRQCFNPMIVELAEKAMKEYGEDPKIETNKFAAICTHLEVCFMYSDGGSKHRFEIIEGRDRIMAWTVVNSICNTTG
VEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIR
QEMASRSLWDSFRQSERAAAELALVPR

0.8 M Succinic Acid pH 7.0, 2 mM MnCl2, 2 mM MgCl2

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

St. Jude Children's Research Hospital (ALSAC)

Virus, Nuclease, Transcription, Cap-snatching, HYDROLASE, HYDROLASE-HYDROLASE INHIBITOR complex

KHRFEIIEGRDRIMAWTVVNSICNTTGVEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSFTG
EEMATKADYTLDEESRARIKTRLFTIRQEMASRSLWDSFRQSER