Solution Structure of XPH2, a Hybrid Sequence of Xfaso 1 and Pfl 6, Two Cro Proteins With Different Folds
Multistep mutational transformation of a protein fold through structural intermediates.
Hybrid between two natural Cro sequences from prophages in Xylella fastidiosa and Pseudomonas fluorescens
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
Water refinement of the lowest energy 20 structures was performed using CNS
0.8 mM [U-13C; U-15N] XPH2, 50 mM NA- potassium phosphate, 150 mM NA- potassium chloride, 0.01 % NA- sodium azide, 0.1 mM NA- DSS, 1 M [U-2H] trimethylamine oxide, 90% H2O/10% D2O
1.6 mM [U-10% 13C] XPH2, 50 mM NA- potassium phosphate, 150 mM NA- potassium chloride, 0.01 % NA- sodium azide, 0.1 mM NA- DSS, 90% H2O/10% D2O
0.9 mM [U-15N] XPH2, 50 mM NA- potassium phosphate, 150 mM NA- potassium chloride, 0.01 % NA- sodium azide, 0.1 mM NA- DSS, 90% H2O/10% D2O
0.6 mM [U-13C; U-15N] XPH2, 50 mM NA- potassium phosphate, 150 mM NA- potassium chloride, 0.01 % NA- sodium azide, 0.1 mM NA- DSS, 1 M [U-2H] trimethylamine oxide, 100% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Monomer confirmed by SEC (gel filtration)
helix-turn-helix, structural evolution, conformational switch, metamorphic protein, disulfide bond, transcription factor, TRANSCRIPTION